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Peptide record

TPDB07280

Fl-Tat HIV-1D-Tat HIV-TAT L-Tat PMM-Tat (conjugate 6) PTD9 (Tat) TAT TAT (48-60) (Compound 5) Tat (48–60) TAT2 Antibacterial Anticancer Cell-penetrating Peptides Toxicity Tumor-homing Peptides standard
13 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB07280
Fl-Tat HIV-1D-Tat HIV-TAT L-Tat PMM-Tat (conjugate 6) PTD9 (Tat) TAT TAT (48-60) (Compound 5) Tat (48–60) TAT2
Antibacterial Anticancer Cell-penetrating Peptides Toxicity Tumor-homing Peptides
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 Peptipedia CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP TumorHoPe2.0
standard
No
A 13-aa standard natural multi-activity (Antibacterial, Anticancer, Cell-penetrating Peptides, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CellPPD, and other sources, with an available 3D structural model.
Sequence
GRKKRRQRRRPPQ
Physicochemical Analysis
C70H131N35O16
ANDCEHILMFSTWYV
R
1719.04
13.10
8
0
0
+8
8.91
-3.492
0.00
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
2
Residue Composition
number
0
A
6
R
0
N
0
D
0
C
0
E
2
Q
1
G
0
H
0
I
0
L
2
K
0
M
0
F
2
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 6 N: 0 D: 0 C: 0 E: 0 Q: 2 G: 1 H: 0 I: 0 L: 0 K: 2 M: 0 F: 0 P: 2 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
13
C70H131N35O16
1719.04
13.10
+8
8.91
-3.492
Free
L
Cationic
Linear
Free
NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)N)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](Cc1ccccc1)C(=O)/N=C/C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CS)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O NCC(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)O N[C@@H](C(C)C)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CS)C(=O)O N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)O
Evidence Records 21 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Porcine skin

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Protein derived
Sodium diclofenac (Na-DFC), Small molecule drug [celecoxib (CLXB)]
High
In vitro
CCCCCCCCCCCCC
Evidence 2 Activity

Activity

Cell-penetrating Peptides

Target

HEK293 And HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
FITC-labelled
Cytosol
In vitro
CCCCCCCCCCCCC
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

Jurkat T Clone E6-1, H9 T Cells, PBMCs And MAGI-CCR5

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
anti-p24 mAb
In vitro
CCCCCCCCCCCCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

Gf-1 Cells

Assay & Model

AB strain of zebrafish
AB strain of zebrafish

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Manganese Superoxide Dismutase (MnSOD)
TAT conjugation increased the internalization of gMnSOD in GF-1 cells.
In vitro and in vivo
CCCCCCCCCCCCC
Evidence 5 Activity

Activity

Cell-penetrating Peptides

Target

S. Aureus Infected Mac-T Or Hbmec Cell Lines , Ex Vivo Calvaria (Skull Cap)

Assay & Model

Femur injury model (Wistar rats (5 to 6 wks of age))
Femur injury model (Wistar rats (5 to 6 wks of age))

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Protein derived
Lysostaphin and LysK
In vitro and in vivo
CCCCCCCC
Evidence 6 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
5(6)-carboxyfluorescein (CF)
In vitro
CCCCCCCCSTTC
Evidence 7 Activity

Activity

Cell-penetrating Peptides

Target

Jurkat Cells And Mouse Primary T Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
AAV6-green fluorescent protein (AAV6-GFP)
In vitro
CCTTSSCCCCC
Evidence 8 Activity

Activity

Cell-penetrating Peptides

Target

Ht22 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Calpain-1 C2-like (C2L) domain
Mitochondria And Other
In vitro
CCTTTSSSSSCCCCCCC
Evidence 9 Activity

Activity

Cell-penetrating Peptides

Target

Gfp-Caveolin-1 In Hct 116 And MCF-7 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Protein derived
Fas-associated death domain (FADD)
Cytosol
5 μM TAT-FADD efficiently internalized across the membrane and was retained in the cytosol of HCT116 cells.
Caveolae mediated endocytosis
In vitro
CCTTSSCCCCC
Evidence 10 Activity

Activity

Cell-penetrating Peptides

Assay & Model

P. falciparum trophozoite stage cells
P. falciparum trophozoite stage cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
SYBR green-I, doxorubicin and actinomycin D
In vivo
CCCCSSCCCC
Evidence 11 Activity

Activity

Cell-penetrating Peptides

Target

Caco-2

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Nanoparticles and FITC labelled dextrans
R8 and Tat increased the uptake of 200-nm nanoparticles by Caco-2 cells to 2.1-3.0 times that was similar or superior than penetratin and PenetraMax
In vitro
CCCCCCCCC
Evidence 12 Activity

Activity

Cell-penetrating Peptides

Target

Nih/3T3, CHO-K1, Or HeLa Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
TfAF488 or 0.2-μm microspheres , lentivirus particles carrying the pac gene
PepB2 show greater efficacy than other
In vitro
CCCCSSCSSSSSSCCC
Evidence 13 Activity

Activity

Cell-penetrating Peptides

Target

HeLa Pluc705 Cells , RAW264.7, Hepg2, And MCF-7 Cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
PNA705, tetramethylrhodamine (TAMRA) and GFP11 peptide
Cytoplasm
Slight 3-fold increase in luminescence over untreated cells and TAMRA delivery follows P17 >>P11 = P14 > P40 = Arg9 > tat = penetratin
Direct translocation and endocytosis
In vitro
CCSCCSSSSCCCCCSCCCCC
Evidence 14 Activity

Activity

Cell-penetrating Peptides

Assay & Model

Male Wistar Han rats
Male Wistar Han rats

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
In vivo
CCTHHHHCC
Evidence 15 Activity

Activity

Cell-penetrating Peptides

Target

HeLa cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
YOYO-1 labeled pDNA
Cytoplasm
Lower uptake for DNA-CPP complexes
Non-endocytic mechanism
In vitro
CCSCSSSCHHHHHHHHHHHHHHTHHHHTCC
Evidence 16 Activity

Activity

Cell-penetrating Peptides

Target

Leishmania donovani promastigotes

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
Paromomycin (PMM) and Bodi Fluor 488 dye
Cytoplasm
In vitro
CCCCCCCCC
Evidence 17 Activity

Activity

Cell-penetrating Peptides

Target

bEnd.3 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
FD-4 molecule and Insulin
Uptake effect of R8 (both L- and D-forms) was stronger than that of Tat
In vitro
CCCTTSSCCSCSGGGTSCC
Evidence 18 Activity

Activity

Cell-penetrating Peptides

Target

Caco-2 cells

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
TAMRA-labeled and iCAL36 peptide
Cytoplasm and Intramembrane
In vitro
CCSCGGGGGGSCC
Evidence 19 Activity

Activity

Cell-penetrating Peptides

Target

HEI-OC1 cells

Assay & Model

Guinea pigs (250–300 g)
Guinea pigs (250–300 g)

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L Cationic
Protein derived
coumarin-6-loaded PLGA-NPs and P407-PLGA-NPs
LMWP group had stronger fluorescence, followed by penetratin, TAT and R8 groups
In vitro and in vivo
CTTGGGTTHHHHHHHHHHHHTTTTHHHHTTSSHHHHHHHHHHHHHHHTTTCC
Evidence 20 Activity

Activity

5% Hemolysis
Toxicity
>200 µM
Hemolytic Cytotoxic
5% Hemolysis | >200 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Effects of dimerization of the cell-penetrating peptide Tat analog on antimicrobial activity and mechanism of bactericidal action. | J Pept Sci | 2009
Evidence 21 Activity

Activity

Tumor-homing Peptides

Target

Tumor cell
Targeting

Source & Reference

TumorHoPe2.0
TumorHoPe2
Insights into the uptake mechanism of NrTP, a cell-penetrating peptide preferentially targeting the nucleolus of tumour cells
2012
Gandhi Rádis-Baptista et al. Chem Biol Drug Des. 2012 Jun 79(6):907-15.

Other

Source Activity Label Source Definition
Tumor-homing Peptides
TumorHoPe2.0 experimentally validated tumor-homing peptide annotation.
Human cervix epithelial carcinoma (HeLa), pancreatic adenocarcinoma (BxPC-3), ductal mammary gland carcinoma (BT-474) and murine neu roblastome (N2A)
Synthetic
Additional Detail Fields 2 fields
CCCCCCCCCCCCC CCCCCCCC CCCCCCCCSTTC CCTTSSCCCCC CCTTTSSSSSCCCCCCC CCCCSSCCCC CCCCCCCCC CCCCSSCSSSSSSCCC CCSCCSSSSCCCCCSCCCCC CCTHHHHCC CCSCSSSCHHHHHHHHHHHHHHTHHHHTCC CCCTTSSCCSCSGGGTSCC CCSCGGGGGGSCC CTTGGGTTHHHHHHHHHHHHTTTTHHHHTTSSHHHHHHHHHHHHHHHTTTCC
Human cervix epithelial carcinoma (HeLa), pancreatic adenocarcinoma (BxPC-3), ductal mammary gland carcinoma (BT-474) and murine neu roblastome (N2A)