Back to search

Peptide record

TPDB07256

Antibacterial Antifungal Antiparasitic Cell-penetrating Peptides Hemolysis Toxicity standard

13 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB07256

Activity

Antibacterial Antifungal Antiparasitic Cell-penetrating Peptides Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia Antifungipept CAFPdb dbAMP 3.0 CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 13-aa standard natural multi-activity (Antibacterial, Antifungal, Antiparasitic, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

ALWKTLLKKVLKA

FASTA

>TPDB07256|Antibacterial;Antifungal;Antiparasitic;Cell-penetrating Peptides;Hemolysis;Toxicity|length=13
ALWKTLLKKVLKA

Formula C74H130N18O15

Absent Amino Acids RNDCEQGHIMFPSY

Common Amino Acids LK

Mass 1511.96

PI 11.00

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +4

Boman Index -0.26

Hydrophobicity 0.446

Aliphatic Index 157.69

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 363.77

Polar Residues 1

number

Amino Acid Distribution

A: 2 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 4 K: 4 M: 0 F: 0 P: 0 S: 0 T: 1 W: 1 Y: 0 V: 1

Length

Formula

C74H130N18O15

Mass

1511.96

11.00

Net Charge

Boman Index

-0.26

Hydrophobicity

0.446

Evidence 1 Activity

Activity

Activity Endpoint LC50

Detail Source Activity Hemolysis

Activity Measurement 50 µM

Hemolysis Raw

[Ref.15917541]LC50=50 µM against human red blood cells

Target

RBC Target human red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

J Biol Chem. 2006 Apr 7 281(14):9432-9438.##Antimicrob Agents Chemother. 2005 Jun 49(6):2412-2420.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 57±3 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 57±3 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Evidence 3 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 21.2±0.6 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 21.2±0.6 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Direct interaction of dermaseptin S4 aminoheptanoyl derivative with intraerythrocytic malaria parasite leading to increased specific antiparasitic activity in culture. | J Biol Chem | 2002

Evidence 4 Activity

Activity

Activity Endpoint 14% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 100 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

14% Hemolysis | 100 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Potent antimicrobial peptides with selectivity for Bacillus anthracis over human erythrocytes. | Int J Antimicrob Agents | 2011

Evidence 5 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >100 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >100 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effects of acyl versus aminoacyl conjugation on the properties of antimicrobial peptides. | Antimicrob Agents Chemother | 2005

Evidence 6 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 25±3 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 25±3 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Physicochemical properties that enhance discriminative antibacterial activity of short dermaseptin derivatives. | Antimicrob Agents Chemother | 2006

Evidence 7 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 6±0.5 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 6±0.5 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Physicochemical properties that enhance discriminative antibacterial activity of short dermaseptin derivatives. | Antimicrob Agents Chemother | 2006

Evidence 8 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 30±2 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 30±2 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Physicochemical properties that enhance discriminative antibacterial activity of short dermaseptin derivatives. | Antimicrob Agents Chemother | 2006

Evidence 9 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 80 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 80 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effects of acyl versus aminoacyl conjugation on the properties of antimicrobial peptides. | Antimicrob Agents Chemother | 2005

Evidence 10 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 75 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 75 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effects of acyl versus aminoacyl conjugation on the properties of antimicrobial peptides. | Antimicrob Agents Chemother | 2005

Evidence 11 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 60 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 60 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effects of acyl versus aminoacyl conjugation on the properties of antimicrobial peptides. | Antimicrob Agents Chemother | 2005

Evidence 12 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 15 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 15 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effects of acyl versus aminoacyl conjugation on the properties of antimicrobial peptides. | Antimicrob Agents Chemother | 2005

Evidence 13 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 4.5 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 4.5 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effects of acyl versus aminoacyl conjugation on the properties of antimicrobial peptides. | Antimicrob Agents Chemother | 2005

Evidence 14 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 9 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 9 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Effects of acyl versus aminoacyl conjugation on the properties of antimicrobial peptides. | Antimicrob Agents Chemother | 2005

Evidence 15 Activity

Activity

Activity Endpoint LC50

Detail Source Activity Toxicity

Activity Measurement 50 µM

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.15917541]LC50=50 µM against human red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

J Biol Chem. 2006 Apr 7 281(14):9432-9438.##Antimicrob Agents Chemother. 2005 Jun 49(6):2412-2420.

Evidence 16 Hemolysis

Activity

Activity Measurement 50 µM

Activity Endpoint LC50

Hemolysis Raw

[Ref.15917541]LC50=50 µM against human red blood cells

Detail Source Activity Hemolysis

Target

RBC Target human red blood cells

Source & Reference

source HemoPI PD_Hemolysis

Reference

J Biol Chem. 2006 Apr 7 281(14):9432-9438.##Antimicrob Agents Chemother. 2005 Jun 49(6):2412-2420.

Source DRAMP4

Database Source PD_Hemolysis