TPDB

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB07233

Activity

Antibacterial Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 13-aa standard natural multi-activity (Antibacterial, Hemolysis, and Toxicity) peptide sequence curated from AntiBP3, dbAMP, Peptipedia, and other sources, with an available 3D structural model.

Sequence

RRWPLKPKKWPLI

FASTA

>TPDB07233|Antibacterial;Hemolysis;Toxicity|length=13
RRWPLKPKKWPLI

Formula C85H136N24O14

Absent Amino Acids ANDCEQGHMFSTYV

Common Amino Acids KP

Mass 1718.17

PI 12.43

Basic Residues 5

Acidic Residues 0

Hydrophobic Residues 5

Net Charge +5

Boman Index 0.81

Hydrophobicity -1.169

Aliphatic Index 90.00

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 11000

Absorbance 280nm 640.22

Polar Residues 0

number

0

A

2

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

1

I

2

L

3

K

0

M

0

F

3

P

0

S

0

T

2

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 2 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 1 L: 2 K: 3 M: 0 F: 0 P: 3 S: 0 T: 0 W: 2 Y: 0 V: 0

Length

13

Formula

C85H136N24O14

Mass

1718.17

PI

12.43

Net Charge

+5

Boman Index

0.81

Hydrophobicity

-1.169

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 12.5 µg/mL

Target

Target Organism B. subtilis IFO 3513

Source & Reference

Database Source dbAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Evidence Text

Active against Gram+ B. subtilis IFO 3513 (MIC 12.5 ug/ml), S. aureus IFO 12732 (MIC 12.5 ug/ml), and Gram- E. coli IFO 12734 (MIC 50 ug/ml).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 12.5 µg/mL

Target

Target Organism Staphylococcus aureus IFO 12732

Source & Reference

Database Source dbAMP

Other

Evidence Text

Active against Gram+ B. subtilis IFO 3513 (MIC 12.5 ug/ml), S. aureus IFO 12732 (MIC 12.5 ug/ml), and Gram- E. coli IFO 12734 (MIC 50 ug/ml).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 50 µg/mL

Target

Target Organism Escherichia coli IFO 12734

Source & Reference

Database Source dbAMP

Other

Evidence Text

m+ B. subtilis IFO 3513 (MIC 12.5 ug/ml), S. aureus IFO 12732 (MIC 12.5 ug/ml), and Gram- E. coli IFO 12734 (MIC 50 ug/ml).

Evidence 4 Activity

Activity

Activity Endpoint 7% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 50 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

7% Hemolysis | 50 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Structure-activity relationship of indolicidin, a Trp-rich antibacterial peptide. | J Pept Sci | 2010

Evidence 5 Hemolysis

Source & Reference

source HemoPI PD_Hemolysis

Database Source PD_Hemolysis