Back to search

Peptide record

TPDB07202

L‐K6 Antibacterial Anticancer Antifungal Cell-penetrating Peptides Toxicity standard

13 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB07202

Peptide_Name

L‐K6

Activity

Antibacterial Anticancer Antifungal Cell-penetrating Peptides Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 Peptipedia CancerPPD 2.0 Antifungipept CAFPdb dbAMP CPPsite3.0

Sequence_Type

standard

Has_Modification No

Description A 13-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CancerPPD 2.0, and other sources, with an available 3D structural model.

Sequence

IKKILSKIKKLLK

FASTA

>TPDB07202|Antibacterial;Anticancer;Antifungal;Cell-penetrating Peptides;Toxicity|length=13
IKKILSKIKKLLK

Formula C75H145N19O15

Absent Amino Acids ARNDCEQGHMFPTWYV

Common Amino Acids K

Mass 1553.09

PI 11.21

Basic Residues 6

Acidic Residues 0

Hydrophobic Residues 6

Net Charge +6

Boman Index 3.46

Hydrophobicity 0.054

Aliphatic Index 180.00

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 1

number

Amino Acid Distribution

A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 3 L: 3 K: 6 M: 0 F: 0 P: 0 S: 1 T: 0 W: 0 Y: 0 V: 0

Length

Formula

C75H145N19O15

Mass

1553.09

11.21

Net Charge

Boman Index

3.46

Hydrophobicity

0.054

C_terminal_Modification

Free

Chirality

Ionic_State

Cationic

Linearity

Linear

N_terminal_Modification

Free

SMILES

N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)O

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 5.00 µM

Target

Target Organism Staphylococcus aureus

Source & Reference

Database Source dbAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Evidence Text

active against Gram+ S. aureus (MIC 5.00 uM), E. faecalis (MIC 3.13 uM), E. faecium (MIC 5.00 uM), B. cereus (MIC 6.25 uM), S. epiderm

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 3.13 µM

Target

Target Organism Enterococcus faecalis

Source & Reference

Database Source dbAMP

Other

Evidence Text

active against Gram+ S. aureus (MIC 5.00 uM), E. faecalis (MIC 3.13 uM), E. faecium (MIC 5.00 uM), B. cereus (MIC 6.25 uM), S. epidermidis (MIC 4.69 uM), Gram- E

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 5.00 µM

Target

Target Organism E. faecium

Source & Reference

Database Source dbAMP

Other

Evidence Text

active against Gram+ S. aureus (MIC 5.00 uM), E. faecalis (MIC 3.13 uM), E. faecium (MIC 5.00 uM), B. cereus (MIC 6.25 uM), S. epidermidis (MIC 4.69 uM), Gram- E. coli (MIC 3.13 uM), P. a

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 6.25 µM

Target

Target Organism B. cereus

Source & Reference

Database Source dbAMP

Other

Evidence Text

ainst Gram+ S. aureus (MIC 5.00 uM), E. faecalis (MIC 3.13 uM), E. faecium (MIC 5.00 uM), B. cereus (MIC 6.25 uM), S. epidermidis (MIC 4.69 uM), Gram- E. coli (MIC 3.13 uM), P. aeruginosa (MIC 6.25 uM),

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4.69 µM

Target

Target Organism Staphylococcus epidermidis

Source & Reference

Database Source dbAMP

Other

Evidence Text

C 5.00 uM), E. faecalis (MIC 3.13 uM), E. faecium (MIC 5.00 uM), B. cereus (MIC 6.25 uM), S. epidermidis (MIC 4.69 uM), Gram- E. coli (MIC 3.13 uM), P. aeruginosa (MIC 6.25 uM), K.pneumoniae (MIC >100 uM), E.

Evidence 6 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 3.13 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source dbAMP

Other

Evidence Text

), E. faecium (MIC 5.00 uM), B. cereus (MIC 6.25 uM), S. epidermidis (MIC 4.69 uM), Gram- E. coli (MIC 3.13 uM), P. aeruginosa (MIC 6.25 uM), K.pneumoniae (MIC >100 uM), E.cloacae (MIC >100 uM), and P.

Evidence 7 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 6.25 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source dbAMP

Other

Evidence Text

uM), B. cereus (MIC 6.25 uM), S. epidermidis (MIC 4.69 uM), Gram- E. coli (MIC 3.13 uM), P. aeruginosa (MIC 6.25 uM), K.pneumoniae (MIC >100 uM), E.cloacae (MIC >100 uM), and P.mirabilis (MIC >100 uM).

Evidence 8 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >100 µM

Target

Target Organism K.pneumoniae

Source & Reference

Database Source dbAMP

Other

Evidence Text

, S. epidermidis (MIC 4.69 uM), Gram- E. coli (MIC 3.13 uM), P. aeruginosa (MIC 6.25 uM), K.pneumoniae (MIC >100 uM), E.cloacae (MIC >100 uM), and P.mirabilis (MIC >100 uM).

Evidence 9 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >100 µM

Target

Target Organism E.cloacae

Source & Reference

Database Source dbAMP

Other

Evidence Text

M), Gram- E. coli (MIC 3.13 uM), P. aeruginosa (MIC 6.25 uM), K.pneumoniae (MIC >100 uM), E.cloacae (MIC >100 uM), and P.mirabilis (MIC >100 uM).

Evidence 10 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >100 µM

Target

Target Organism P.mirabilis

Source & Reference

Database Source dbAMP

Other

Evidence Text

M), P. aeruginosa (MIC 6.25 uM), K.pneumoniae (MIC >100 uM), E.cloacae (MIC >100 uM), and P.mirabilis (MIC >100 uM).

Evidence 11 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 5.00 µM

Target

Target Organism Staphylococcus aureus

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class.

Evidence Text

active against Gram+ S. aureus (MIC 5.00 uM), E. faecalis (MIC 3.13 uM), E. faecium (MIC 5.00 uM), B. cereus (MIC 6.25 uM), S. epiderm

Evidence 12 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 3.13 µM

Target

Target Organism Enterococcus faecalis

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

active against Gram+ S. aureus (MIC 5.00 uM), E. faecalis (MIC 3.13 uM), E. faecium (MIC 5.00 uM), B. cereus (MIC 6.25 uM), S. epidermidis (MIC 4.69 uM), Gram- E

Evidence 13 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 5.00 µM

Target

Target Organism E. faecium

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

active against Gram+ S. aureus (MIC 5.00 uM), E. faecalis (MIC 3.13 uM), E. faecium (MIC 5.00 uM), B. cereus (MIC 6.25 uM), S. epidermidis (MIC 4.69 uM), Gram- E. coli (MIC 3.13 uM), P. a

Evidence 14 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 6.25 µM

Target

Target Organism B. cereus

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

Evidence 15 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 4.69 µM

Target

Target Organism Staphylococcus epidermidis

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

Evidence 16 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 3.13 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

Evidence 17 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 6.25 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

Evidence 18 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement >100 µM

Target

Target Organism K.pneumoniae

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

, S. epidermidis (MIC 4.69 uM), Gram- E. coli (MIC 3.13 uM), P. aeruginosa (MIC 6.25 uM), K.pneumoniae (MIC >100 uM), E.cloacae (MIC >100 uM), and P.mirabilis (MIC >100 uM).

Evidence 19 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement >100 µM

Target

Target Organism E.cloacae

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

M), Gram- E. coli (MIC 3.13 uM), P. aeruginosa (MIC 6.25 uM), K.pneumoniae (MIC >100 uM), E.cloacae (MIC >100 uM), and P.mirabilis (MIC >100 uM).

Evidence 20 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement >100 µM

Target

Target Organism P.mirabilis

Source & Reference

Database Source dbAMP CAFPdb Antifungipept

Other

Evidence Text

M), P. aeruginosa (MIC 6.25 uM), K.pneumoniae (MIC >100 uM), E.cloacae (MIC >100 uM), and P.mirabilis (MIC >100 uM).

Evidence 21 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Protein derived

Cargo Type EGFP protein

Subcellular Localization Cytoplasm

Uptake Efficiency

EGFP‐CAT 2‐8‐times higher intensity than other derivative and 3‐6 times higher than EGFP‐LL37, EGFP‐IL, EGFP‐L‐K6, and EGFP‐TAT

In Vitro or In Vivo In vitro

DSSP Secondary Structure CHHHHHHHHHHTC

Evidence 22 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 29.97 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 29.97 | µg/mL | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Lipidation of Naturally Occurring α-Helical Antimicrobial Peptides as a Promising Strategy for Drug Design | Int J Mol Sci | 2023

Evidence 23 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement >500 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | >500 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Antimicrobial properties and interaction of two Trp-substituted cationic antimicrobial peptides with a lipid bilayer. | J Antibiot (Tokyo) | 2014

Evidence 24 Activity

Activity

Activity Endpoint <5% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 512 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

<5% Hemolysis | 512 | µg/mL | target cell: Sheep erythrocytes

Target

Target Cell Sheep erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Impact of lipidation site on the activity of α-helical antimicrobial peptides | Bioorg Chem | 2024

Evidence 25 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Cytotoxic

Toxicity Raw

NA | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Impact of lipidation site on the activity of α-helical antimicrobial peptides | Bioorg Chem | 2024

Evidence 26 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 65.88

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Rabbit red blood cells: modest hemolytic activity(as show in Fig.2) | Cytotoxicity: HUVEC: IC50=65.88±4.5 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Sci Rep. 2017 Aug 15 7(1):8293.

DSSP_Secondary_Structure

CHHHHHHHHHHTC