TPDB

3D PDB MODEL

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TPDB ID TPDB07133

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia CancerPPD 2.0 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 13-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CancerPPD 2.0, and other sources, with an available 3D structural model.

Sequence

ILPWKWPWWPWKK

FASTA

>TPDB07133|Antibacterial;Anticancer;Hemolysis;Toxicity|length=13
ILPWKWPWWPWKK

Formula C100H131N21O14

Absent Amino Acids ARNDCEQGHMFSTYV

Common Amino Acids W

Mass 1851.27

PI 10.84

Basic Residues 3

Acidic Residues 0

Hydrophobic Residues 7

Net Charge +3

Boman Index -5.80

Hydrophobicity -0.977

Aliphatic Index 60.00

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 27500

Absorbance 280nm 1485.46

Polar Residues 0

number

0

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

1

I

1

L

3

K

0

M

0

F

3

P

0

S

0

T

5

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 1 L: 1 K: 3 M: 0 F: 0 P: 3 S: 0 T: 0 W: 5 Y: 0 V: 0

Length

13

Formula

C100H131N21O14

Mass

1851.27

PI

10.84

Net Charge

+3

Boman Index

-5.80

Hydrophobicity

-0.977

Evidence 1 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Hemolysis

Activity Measurement 65 µM

Hemolysis Raw

RBC: IC50 ≥ 65 µM

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Biochim Biophys Acta Biomembr. 2020 Aug 1 1862(8):183228.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 100% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 31.3 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

100% Hemolysis | 31.3 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Structure-function relationships in the tryptophan-rich, antimicrobial peptide indolicidin. | J Pept Sci | 2001

Evidence 3 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 26.4 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 26.4 | µM | target cell: Human PBMC

Target

Target Cell Human PBMC

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Selective anticancer activity of synthetic peptides derived from the host defence peptide tritrpticin. | Biochim Biophys Acta | 2020

Evidence 4 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 65 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: RBC: IC50 ≥ 65 µM | Cytotoxicity: PBMC: IC50=26.4 µM

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Biochim Biophys Acta Biomembr. 2020 Aug 1 1862(8):183228.

Evidence 5 Hemolysis

Activity

Activity Measurement 65 µM

Activity Endpoint IC50

Hemolysis Raw

RBC: IC50 ≥ 65 µM

Detail Source Activity Hemolysis

Source & Reference

source PD_Hemolysis

Reference

Biochim Biophys Acta Biomembr. 2020 Aug 1 1862(8):183228.

Source DRAMP4

Database Source PD_Hemolysis