TPDB

3D PDB MODEL

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TPDB ID TPDB07012

Activity

Antibacterial Anticancer Antifungal Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia CancerPPD 2.0 Antifungipept CAFPdb

Sequence_Type

standard

Has_Modification No

Description A 13-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

FLYIVAKLLSGLL

FASTA

>TPDB07012|Antibacterial;Anticancer;Antifungal;Toxicity|length=13
FLYIVAKLLSGLL

Formula C73H120N14O16

Absent Amino Acids RNDCEQHMPTW

Common Amino Acids L

Mass 1449.84

PI 9.74

Basic Residues 1

Acidic Residues 0

Hydrophobic Residues 10

Net Charge +1

Boman Index -4.15

Hydrophobicity 1.992

Aliphatic Index 210.00

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 102.77

Polar Residues 2

number

1

A

0

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

1

I

5

L

1

K

0

M

1

F

0

P

1

S

0

T

0

W

1

Y

1

V

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 1 L: 5 K: 1 M: 0 F: 1 P: 0 S: 1 T: 0 W: 0 Y: 1 V: 1

Length

13

Formula

C73H120N14O16

Mass

1449.84

PI

9.74

Net Charge

+1

Boman Index

-4.15

Hydrophobicity

1.992

Evidence 1 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 40.30 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 40.30 | µM | target cell: Human microvascular endothelial cells HMEC-1

Target

Target Cell Human microvascular endothelial cells HMEC-1

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. esculentus. | Biochem Biophys Res Commun | 2017

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 526.5 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 526.5 | µM | target cell: Horse erythrocytes

Target

Target Cell Horse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Identification and target-modifications of temporin-PE: A novel antimicrobial peptide in the defensive skin secretions of the edible frog, Pelophylax kl. esculentus. | Biochem Biophys Res Commun | 2017

Evidence 3 Activity

Activity

Activity Endpoint HC50

Detail Source Activity Toxicity

Activity Measurement 531.7 µM

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.29191658]HC50=531.7 µM against horse red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Biochem Biophys Res Commun. 2018 Jan 22 495(4):2539-2546.