Peptide record

TPDB05636

Arg8 cyclo R8 L-R8 l-R8-C6-NP L-R8-INS-NP Mn1-R9 octa-arginine octa-arginine (R8) Octa-arginine(R8) octaarginine (R8) R8-lip R9-GFP CR8 P4 PTD8 R8 Antibacterial Antiviral Blood-Brain Barrier Cell-penetrating Peptides Tumor-homing Peptides standard

8 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB05636

Peptide_Name

Arg8 cyclo R8 L-R8 l-R8-C6-NP L-R8-INS-NP Mn1-R9 octa-arginine octa-arginine (R8) Octa-arginine(R8) octaarginine (R8) R8-lip R9-GFP CR8 P4 PTD8 R8

Activity

Antibacterial Antiviral Blood-Brain Barrier Cell-penetrating Peptides Tumor-homing Peptides

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Delivery and barrier-penetrating peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia BBPpredict CellPPD CellPPD-MOD CPPsite3.0 PerseuCPP TumorHoPe2.0

Sequence_Type

standard

Has_Modification No

Description A 8-aa standard natural multi-activity (Antibacterial, Antiviral, Blood-Brain Barrier, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

RRRRRRRR

FASTA

>TPDB05636|Antibacterial;Antiviral;Blood-Brain Barrier;Cell-penetrating Peptides;Tumor-homing Peptides|length=8
RRRRRRRR

Formula C48H98N32O9

Absent Amino Acids ANDCEQGHILKMFPSTWYV

Common Amino Acids R

Mass 1267.52

PI 13.25

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 0

Net Charge +8

Boman Index 6.48

Hydrophobicity -4.500

Aliphatic Index 0.00

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 0

number

Amino Acid Distribution

A: 0 R: 8 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0

Length

Formula

C48H98N32O9

Mass

1267.52

13.25

Net Charge

Boman Index

6.48

Hydrophobicity

-4.500

C_terminal_Modification

Free

Chirality

Ionic_State

Cationic Amphipathic Cationic and hydrophobic

Linearity

Linear Cyclic

N_terminal_Modification

Free

SMILES

N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](CC1=NC=NC1)C(=O)O N[C@@H](Cc1ccccc1)C(=O)/N=C/C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)O N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](C(C)C)C(=O)O NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O NCC(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CCSC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(=O)N)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)O NCC(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)NCC(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](Cc1ccccc1)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)O N[C@@H](CS)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CS)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CS)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)NCC(=O)N[C@@H](CC(=O)N)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CS)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)NCC(=O)/N=C/C(=O)N[C@@H](C(C)C)C(=O)O NCC(=O)NCC(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)O NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)O N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)O N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)O

Evidence 1 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Assay & Model

In Vitro Model Synechocystis sp. PCC 6803, Synechococcus elongatus PCC 7942

In Vivo Model Synechocystis sp. PCC 6803, Synechococcus elongatus PCC 7942

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

Source Activity Label	Source Definition
Cell-penetrating Peptides	CPPsite3.0 experimentally validated cell-penetrating peptide annotation.

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Protein (eGFP)

Subcellular Localization Cytoplasm

Uptake Mechanism Endocytosis (Macropinocytosis)

In Vitro or In Vivo In vivo

DSSP Secondary Structure CCCCCCCC

Evidence 2 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line 293T and HeLa

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Amphipathic

Source of Peptide Synthetic

Cargo Type Nucleic acid (Plasmid DNA)

Subcellular Localization Nucleus

Uptake Mechanism Endocytic pathway

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 3 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Bone marrow dendritic cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Nanoparticle (Liposomes)

Subcellular Localization Cytosol

Uptake Efficiency R8-Lip resulted in a drastic enhancement in the cross-presentation of the OVA antigen compared to K8-Lip

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 4 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa cells

Assay & Model

In Vitro Model Female Kunming (KM) mice

In Vivo Model Female Kunming (KM) mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Fluorophore (FITC)

Subcellular Localization Vesicles

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCCCC

Evidence 5 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Nanoparticle (Couramin-6 loaded nanoparticle)

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 6 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Nanoparticle (Insulin loaded nanoparticle)

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 7 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HUVEC and A549 cells

Assay & Model

In Vitro Model A549 xenografts bearing mice and U87 xenografts bearing mice

In Vivo Model A549 xenografts bearing mice and U87 xenografts bearing mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Nanoparticle (PEG-PCL)

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCCCCCC

Evidence 8 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Rat Primary Cortical Cultures

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Uptake Mechanism Heparan sulfate receptor-mediated endocytic pathway

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCC

Evidence 9 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Intestinal Loops Of Sprague Dawley Rats

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Insulin

Uptake Efficiency L- and D-PenetraMax show greatest increase in ileum insulin absorption than other CPP

In Vitro or In Vivo In Situ

DSSP Secondary Structure CCCCCCCC

Evidence 10 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type FITC-labeled

Subcellular Localization Cytosol, Nucleus and Endosomes

Uptake Efficiency Show considerably lower uptake

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCGGGTSCC

Evidence 11 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line S. Aureus Infected Mac-T Or Hbmec Cell Lines , Ex Vivo Calvaria (Skull Cap)

Assay & Model

In Vitro Model Femur injury model (Wistar rats (5 to 6 wks of age))

In Vivo Model Femur injury model (Wistar rats (5 to 6 wks of age))

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Lysostaphin and LysK

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCSSCGGGHHHHSCCC

Evidence 12 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 Cells

Assay & Model

In Vitro Model Male Ddy mice weighing 30-40 g Ex-vivo ileum closed loop of Sprague Dawley rats weighing 180–220 g

In Vivo Model Male Ddy mice weighing 30-40 g Ex-vivo ileum closed loop of Sprague Dawley rats weighing 180–220 g

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Insulin

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure C

Evidence 13 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Human Mesenchymal Stem Cells (Mscs) Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type Fluorescent polymer dots (Pdots)

Subcellular Localization Cytoplasm

Uptake Efficiency ~3 times higher than the cell brightness labelled with R8-Pdots in MSCs cells

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCTTSSCCCCC

Evidence 14 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hek293T And Ct26.Cl25 Cell Lines

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type 5-methoxyuridine-modified mCherry mRNA, Enhanced green fluorescence protein (EGFP) mRNA and Ovalbumin (OVA) mRNA

Subcellular Localization Cytoplasm

Uptake Efficiency Exhibited poor uptake efficiency with 11.6% for Arg8

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCSCHHHHHHHHSCC

Evidence 15 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa, A549,Caco-2, THP-1, and RAW264.7 cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Salmonella phage selz

Subcellular Localization Late Endosomes/Lysosomes

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCSSCSCCGGGCC

Evidence 16 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Ht29-Md2 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic and hydrophobic

Source of Peptide Synthetic

Cargo Type Superoxide dismutases mimic (Mn1)

Subcellular Localization Cell Periphery

Uptake Efficiency (Mn1-R9 < Mn1-MPP < Mn1-RW9 < Mn1) , Mn content accumluated was 0.56 nmol.mg-1.

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCHHHHHHHTTC

Evidence 17 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2, Hepg2, Hep3B, And Hek 293T Cell Lines

Assay & Model

In Vitro Model Balb/c mice (8 weeks old)

In Vivo Model Balb/c mice (8 weeks old)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Cyclic L Cationic

Source of Peptide Synthetic

Cargo Type Dnase1 and AR (E35K/A136K)

Uptake Efficiency CR8 conjugation helps Dnase1 penetrate into the rectal mucosa.

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCTTTCSTTTSCHHHHTTCCC

Evidence 18 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Gh4C1 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Cyclic L Cationic

Source of Peptide Synthetic

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCCCC

Evidence 19 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 Cells And Insulin Reporter Ilite Cells

Assay & Model

In Vitro Model 12-week-old Male HsdOla:TO mice

In Vivo Model 12-week-old Male HsdOla:TO mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Insulin-GET nanocomplexes (NCs)

Uptake Efficiency transcytosis efficiency of the optimal Insulin-GET NC formulation was ~22-fold greater than the Insulin alone

Uptake Mechanism Energy-dependent transcytosis

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCBTTBCGGGCCSCCCCCCCC

Evidence 20 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line THP-1 Cells Enterococcus Faecalis Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type TAMRA-labelled OP145

Subcellular Localization Macrophage'S Lysosomes And The Cytosol

Uptake Efficiency At 2h incubation, internalization of OP145-R8 into the macrophages was 77.13%.

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCCCTTSCCCCCC

Evidence 21 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Aspc-1, Bxpc-1, Panc-1 , Aspc-1 And Bxpc-3 Cells

Assay & Model

In Vitro Model Female BALB/c nude mice, aged 5–6 weeks with weight of 16–18 g

In Vivo Model Female BALB/c nude mice, aged 5–6 weeks with weight of 16–18 g

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type TRAIL protein at c terminal

Subcellular Localization Cytoplasm And Nuclear Envelope

Uptake Efficiency Found the distribution of TRAIL-Mu3 in the cytoplasm and even in the nuclear envelope of AsPC-1 cell membrane.

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCTTSSCCCCC

Evidence 22 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Nih3T3 And HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Curcumin

Uptake Efficiency

The addition of octaarginine enables curcumin to enter into fibroblasts cells NIH3T3 and HeLa cells. The R8-treated cells show red fluorescence in the PI channel, whereas curcumin-treated cells do not display any signal in the PI channel

Uptake Mechanism Membrane damage

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSCCCCCCC

Evidence 23 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type B1MLM peptide

Subcellular Localization Cytosol

Uptake Efficiency

Fluo4 intensity sharply increased within a few minutes, and it was maintained without the oscillation of cytosolic calcium levels.

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSCCTTGGGGTTTTSCSCCC

Evidence 24 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Bbecs Or Rat Astrocytes And Intestine (Caco-2 Assay) And A Bbb Model

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type carboxyfuorescein (Cf) at N -terminal

Uptake Efficiency Three CPPs (TAT, penetratin and R8) showed the highest internalization profiles in both cell types.

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 25 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Hep G2 And A549 Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Cy3 and FAM-labeled siRNAs

Subcellular Localization Cytoplasm and nucleus

Uptake Efficiency R8 complexes had moderate cellular uptake.

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSCCCC

Evidence 26 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa Cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Fluorescent polymer dots (Pdots)

Subcellular Localization Perinuclear Region (In Cytoplasm)

Uptake Efficiency

Fluorescent signals appear on the cell membrane within 5 minutes, while cells incubated with unmodified Pdots show neglectable fluorescence.

Uptake Mechanism Caveolae mediated endocytosis

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCCC

Evidence 27 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Human Mesenchymal Stem Cells (Mscs)

Assay & Model

In Vitro Model ICR male mice

In Vivo Model ICR male mice

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Semiconducting polymer dots (Pdots)

Subcellular Localization Perinuclear

Uptake Efficiency

Pdots coated with R8 showed remarkable endocytic uptake efficiency that was 15 times higher than that of carboxyl Pdots and more than 200 times than that of bare Pdots.

Uptake Mechanism Endocytosis

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CTTTTCCTTTTGGGTCC

Evidence 28 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2 Cell Monolayer

Assay & Model

In Vitro Model Male Wistar rats

In Vivo Model Male Wistar rats

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Insulin

Uptake Efficiency Transport was in this order: SAR6EW-insulin > R6EW-insulin > R8-insulin > insulin.

Uptake Mechanism Energy dependent pathway route and direct penetration

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCGGGGGTCCCCCCTTCCCCCCTTSSSCSSCSCC

Evidence 29 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line Caco-2

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Nanoparticles and FITC labelled dextrans

Uptake Efficiency

R8 and Tat increased the uptake of 200-nm nanoparticles by Caco-2 cells to 2.1-3.0 times that was similar or superior than penetratin and PenetraMax

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSTTCCCCCC

Evidence 30 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line

Caco-2 Cells Bacteroidetes (Bacteroides Vulgatus And Bacteroides Thetaiotaomicron), Firmicutes (Lactobacillus Gasseri, Latilactobacillus Sakei, And Clostridium Bolteae), Actinomicetya (Bifidobacterium Longum, And Bifidobacterium Adolescentis) , Proteobacteria (Escherichia Coli Nissle 1917 And Escherichia Coli K12) And Saccharomyces Boulardii

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L

Source of Peptide Synthetic

Cargo Type FITC-dextran

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCSHHHHHHHHHHHHTTSCC

Evidence 31 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HeLa cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Alexa Fluor 594 and FITC dye

Subcellular Localization Cytosol

Uptake Mechanism Direct membrane penetration

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCCSCSCCCCCC

Evidence 32 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line LO2 (Human liver cell)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

In Vitro or In Vivo In vitro

DSSP Secondary Structure CCGGGTSCC

Evidence 33 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line bEnd.3 cells

Assay & Model

In Vitro Model Male Sprague-Dawley rats (180–220 g)

In Vivo Model Male Sprague-Dawley rats (180–220 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type FD-4 molecule and Insulin

Uptake Efficiency Uptake effect of R8 (both L- and D-forms) was stronger than that of Tat

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCCSSSTTCCCTTTTC

Evidence 34 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line MCF7 cells

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Non-natural residues Linear L

Source of Peptide Synthetic

Cargo Type HF488 labelled PNAs with 2-aminopyridine (M)

Subcellular Localization Cytoplasm and nucleoli

Uptake Efficiency Strong fluorescence of PNA-Tat and PNA-R8 than PNA-MK3

In Vitro or In Vivo In vitro

Evidence 35 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line U87 and GL261 cells

Assay & Model

In Vitro Model Balb/c Nude mice (male, 6–8 weeks old, 18.0–20.0 g)

In Vivo Model Balb/c Nude mice (male, 6–8 weeks old, 18.0–20.0 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type Transferrin co-modified doxorubicin (DOX)-loaded liposomes (Tf-LPs)

Subcellular Localization Nuclei

Uptake Efficiency Improved cellular efficiency than free DOX and LPs,

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CCGGGTSCC

Evidence 36 Activity

Activity

Detail Source Activity Cell-penetrating Peptides

Target

Cell Line HEI-OC1 cells

Assay & Model

In Vitro Model Guinea pigs (250–300 g)

In Vivo Model Guinea pigs (250–300 g)

Source & Reference

Database Source CPPsite3.0

Source CPPsite3

Other

CPP Category Natural residues Linear L Cationic

Source of Peptide Synthetic

Cargo Type coumarin-6-loaded PLGA-NPs and P407-PLGA-NPs

Uptake Efficiency LMWP group had stronger fluorescence, followed by penetratin, TAT and R8 groups

In Vitro or In Vivo In vitro and in vivo

DSSP Secondary Structure CTTHHHHHHHHHHHHHHHHHHHHHTTHHHHSGGGTTHHHHHHHHHHHHHHHHHHHHHHHHHHHTTTTTTTSSSSTTCTTTHHHHHHHHHHHHHHHHHHHHHHHHTTTCC

Evidence 37 Activity

Activity

Detail Source Activity Tumor-homing Peptides

Target

Homing or Targeting Targeting, Penetrating

Target Tumor Lung cancer

Receptors Biomarker Neuropilin-1

Source & Reference

Database Source TumorHoPe2.0

Source TumorHoPe2

Title Tumor homing cell penetrating peptide decorated nanoparticles used for enhancing tumor targeting delivery and therapy

Year 2014

Reference

Gao H et al. Int J Pharm.2015 Jan 15 478(1):240-250

Other

Source Activity Label	Source Definition
Tumor-homing Peptides	TumorHoPe2.0 experimentally validated tumor-homing peptide annotation.

InVitro ExVivo HUVEC, A549, U87 (Human Glioma cell line)

InVivo Model 4-5 weeks old BALB/c male nude mice

Source Type Synthetic

Conjugate Payload Docetaxel(DTX)

Conjugate_Payload

Docetaxel(DTX)

DSSP_Secondary_Structure

CCCCCCCC CCGGGTSCC CCSSCGGGHHHHSCCC C CCTTSSCCCCC CCCCCSCHHHHHHHHSCC CCCCSSCSCCGGGCC CCHHHHHHHTTC CCTTTCSTTTSCHHHHTTCCC CCCCCCCCC CCCBTTBCGGGCCSCCCCCCCC CCCCCCTTSCCCCCC CCCSCCCCCCC CCSCCTTGGGGTTTTSCSCCC CCCSSSTTCCCTTTTC CCSCCCC CCCCC CTTTTCCTTTTGGGTCC CCGGGGGTCCCCCCTTCCCCCCTTSSSCSSCSCC CCCSTTCCCCCC CCSHHHHHHHHHHHHTTSCC CCCSCSCCCCCC CTTHHHHHHHHHHHHHHHHHHHHHTTHHHHSGGGTTHHHHHHHHHHHHHHHHHHHHHHHHHHHTTTTTTTSSSSTTCTTTHHHHHHHHHHHHHHHHHHHHHHHHTTTCC

InVitro_ExVivo

HUVEC, A549, U87 (Human Glioma cell line)

InVivo_Model

4-5 weeks old BALB/c male nude mice