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Peptide record

TPDB05446

Cliotide T4 Antibacterial Anticancer Antifungal Antiparasitic Antiviral Hemolysis Toxicity standard

30 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB05446

Peptide_Name

Cliotide T4

Activity

Antibacterial Anticancer Antifungal Antiparasitic Antiviral Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF Antifungipept AI4AVP APD_complete APD_hemolytic HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 30-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

GIPCGESCVFIPCITAAIGCSCKSKVCYRN

FASTA

>TPDB05446|Antibacterial;Anticancer;Antifungal;Antiparasitic;Antiviral;Hemolysis;Toxicity|length=30
GIPCGESCVFIPCITAAIGCSCKSKVCYRN

Formula C132H216N36O39S6

Absent Amino Acids DQHLMW

Common Amino Acids C

Mass 3123.74

PI 8.02

Basic Residues 3

Acidic Residues 1

Hydrophobic Residues 10

Net Charge +2

Boman Index 1.74

Hydrophobicity 0.657

Aliphatic Index 78.00

Half Life

Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 1865

Absorbance 280nm 59.70

Polar Residues 12

number

Amino Acid Distribution

A: 2 R: 1 N: 1 D: 0 C: 6 E: 1 Q: 0 G: 3 H: 0 I: 4 L: 0 K: 2 M: 0 F: 1 P: 2 S: 3 T: 1 W: 0 Y: 1 V: 2

Length

Formula

C132H216N36O39S6

Mass

3123.74

8.02

Net Charge

Boman Index

1.74

Hydrophobicity

0.657

C_terminal_Modification

Free

Chirality

Linear_or_Cyclic

Not Available

N_terminal_Modification

Free

Nature

Antibacterial

Origin

Clitoria ternatea

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1.3 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source dbAMP DRAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1.9 µM

Target

Target Organism K. pneumonia

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 1.5 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 4 Activity

Activity

Activity Raw

Not Available

Detail Source Activity Anticancer

Target

Cancer Type Not Available

Cell Line Not Available

Tissue Affected Not Available

Assay & Model

Assay Not Available

Testing Time Not Available

Source & Reference

Database Source CancerPPD 2.0 dbACP SATPdb

Source CancerPPD2

Year 2011

Other

Source Activity Label	Source Definition
Anticancer	CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation.

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 1.3 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source dbAMP DRAMP Antifungipept

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class. DRAMP antifungal activity supported by activity records against fungal target organisms.

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 6 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 1.9 µM

Target

Target Organism K. pneumonia

Source & Reference

Database Source dbAMP DRAMP Antifungipept

Other

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 7 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 1.5 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source dbAMP DRAMP Antifungipept

Other

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 8 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 1.3 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source DRAMP

Other

Source Activity Label	Source Definition
Antiparasitic	DRAMP antiparasitic activity supported by activity records against parasitic targets.

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 9 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 1.9 µM

Target

Target Organism K. pneumonia

Source & Reference

Database Source DRAMP

Other

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 10 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 1.5 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source DRAMP

Other

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 11 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiviral

Activity Measurement 1.3 µM

Target

Target Organism Escherichia coli

Source & Reference

Database Source DRAMP

Other

Source Activity Label	Source Definition
Antiviral	DRAMP antiviral activity supported by activity records against viral targets.

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 12 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiviral

Activity Measurement 1.9 µM

Target

Target Organism K. pneumonia

Source & Reference

Database Source DRAMP

Other

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 13 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiviral

Activity Measurement 1.5 µM

Target

Target Organism Pseudomonas aeruginosa

Source & Reference

Database Source DRAMP

Other

Evidence Text

Active against E. coli (MIC 1.3 uM), K. pneumonia (MIC 1.9 uM), and P. aeruginosa (MIC 1.5 uM).

Evidence 14 Activity

Activity

Activity Endpoint HD50

Detail Source Activity Hemolysis

Activity Measurement 7.1 µM

Hemolysis Raw

[Ref.21596752] HD50 = 7.1 µM against human red blood cells

Target

RBC Target human red blood cells

Source & Reference

Database Source Hemolytik APD_complete APD_hemolytic PD_Hemolysis

Source DRAMP4

Reference

J Biol Chem. 2011 Jul 8 286(27):24275-24287.

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation. Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 15 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 8.4 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 8.4 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family. | J Biol Chem | 2011

Evidence 16 Activity

Activity

Activity Endpoint HD50

Detail Source Activity Toxicity

Activity Measurement 7.1 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: [Ref.21596752] HD50 = 7.1 µM against human red blood cells | Cytotoxicity: [Ref.21596752] IC50=0.6 µM against HeLa cells.

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

J Biol Chem. 2011 Jul 8 286(27):24275-24287.

Evidence 17 Hemolysis

Activity

Activity Measurement 7.1 µM

Activity Endpoint HD50

Hemolysis Raw

[Ref.21596752] HD50 = 7.1 µM against human red blood cells

Detail Source Activity Hemolysis

Target

RBC Target human red blood cells

Source & Reference

source APD_complete APD_hemolytic HemoPI PD_Hemolysis

Reference

J Biol Chem. 2011 Jul 8 286(27):24275-24287.

Source DRAMP4

Database Source APD_complete APD_hemolytic PD_Hemolysis

Other

Source Activity Label	Source Definition
Hemolysis	APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation.