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Peptide record

TPDB05220

Antibacterial Anticancer Antifungal Antiparasitic Antiviral Toxicity standard

18 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB05220

Activity

Antibacterial Anticancer Antifungal Antiparasitic Antiviral Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia AntiCP 2.0 iACP-DRLF Antifungipept CAFPdb AI4AVP dbAMP 3.0 ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 18-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

RRWCFRVCYKGFCYRKCR

FASTA

>TPDB05220|Antibacterial;Anticancer;Antifungal;Antiparasitic;Antiviral;Toxicity|length=18
RRWCFRVCYKGFCYRKCR

Formula C108H164N36O21S4

Absent Amino Acids ANDEQHILMPST

Common Amino Acids R

Mass 2430.96

PI 10.36

Basic Residues 7

Acidic Residues 0

Hydrophobic Residues 6

Net Charge +7

Boman Index -0.84

Hydrophobicity -0.800

Aliphatic Index 16.11

Half Life

Mammalian: 1 hour Yeast: 2 min E.coli: 2 min

Extinction Coefficient Cystines 8730

Absorbance 280nm 359.12

Polar Residues 6

number

Amino Acid Distribution

A: 0 R: 5 N: 0 D: 0 C: 4 E: 0 Q: 0 G: 1 H: 0 I: 0 L: 0 K: 2 M: 0 F: 2 P: 0 S: 0 T: 0 W: 1 Y: 2 V: 1

Length

Formula

C108H164N36O21S4

Mass

2430.96

10.36

Net Charge

Boman Index

-0.84

Hydrophobicity

-0.800

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 3.1-6.3 µg/mL

Target

Target Organism S. typhimurium LT2 and 1102

Source & Reference

Database Source dbAMP DRAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.

Evidence Text

Active against Gram- S. typhimurium LT2 and 1102 (MIC 3.1-6.3 ug/ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S.

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 12.5 µg/mL

Target

Target Organism Escherichia coli K12

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

Active against Gram- S. typhimurium LT2 and 1102 (MIC 3.1-6.3 ug/ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 3.1-12.5 µg/mL

Target

Target Organism S. Minnesota 1114W or R595

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

inst Gram- S. typhimurium LT2 and 1102 (MIC 3.1-6.3 ug/ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC 6.3-12.5 ug/ml), and C. albicans M9 (MIC 6.3 ug/

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 6.3-12.5 µg/mL

Target

Target Organism Staphylococcus aureus 209P or ATCC 25923

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC 6.3-12.5 ug/ml), and C. albicans M9 (MIC 6.3 ug/ml).

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 6.3 µg/mL

Target

Target Organism Candida albicans M9

Source & Reference

Database Source dbAMP DRAMP

Other

Evidence Text

r R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC 6.3-12.5 ug/ml), and C. albicans M9 (MIC 6.3 ug/ml).

Evidence 6 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 3.1-6.3 µg/mL

Target

Target Organism S. typhimurium LT2 and 1102

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class. DRAMP antifungal activity supported by activity records against fungal target organisms.

Evidence Text

Active against Gram- S. typhimurium LT2 and 1102 (MIC 3.1-6.3 ug/ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S.

Evidence 7 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 12.5 µg/mL

Target

Target Organism Escherichia coli K12

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

Active against Gram- S. typhimurium LT2 and 1102 (MIC 3.1-6.3 ug/ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC

Evidence 8 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 3.1-12.5 µg/mL

Target

Target Organism S. Minnesota 1114W or R595

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

Evidence 9 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 6.3-12.5 µg/mL

Target

Target Organism Staphylococcus aureus 209P or ATCC 25923

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC 6.3-12.5 ug/ml), and C. albicans M9 (MIC 6.3 ug/ml).

Evidence 10 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 6.3 µg/mL

Target

Target Organism Candida albicans M9

Source & Reference

Database Source dbAMP DRAMP CAFPdb Antifungipept

Other

Evidence Text

r R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC 6.3-12.5 ug/ml), and C. albicans M9 (MIC 6.3 ug/ml).

Evidence 11 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 3.1-6.3 µg/mL

Target

Target Organism S. typhimurium LT2 and 1102

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Source Activity Label	Source Definition
Antiparasitic	DRAMP antiparasitic activity supported by activity records against parasitic targets. dbAMP 3.0 classified under the Anti-parasitic function class.

Evidence Text

Active against Gram- S. typhimurium LT2 and 1102 (MIC 3.1-6.3 ug/ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S.

Evidence 12 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 12.5 µg/mL

Target

Target Organism Escherichia coli K12

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Evidence Text

Active against Gram- S. typhimurium LT2 and 1102 (MIC 3.1-6.3 ug/ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC

Evidence 13 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 3.1-12.5 µg/mL

Target

Target Organism S. Minnesota 1114W or R595

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Evidence Text

Evidence 14 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 6.3-12.5 µg/mL

Target

Target Organism Staphylococcus aureus 209P or ATCC 25923

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Evidence Text

ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC 6.3-12.5 ug/ml), and C. albicans M9 (MIC 6.3 ug/ml).

Evidence 15 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiparasitic

Activity Measurement 6.3 µg/mL

Target

Target Organism Candida albicans M9

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Evidence Text

r R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC 6.3-12.5 ug/ml), and C. albicans M9 (MIC 6.3 ug/ml).

Evidence 16 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiviral

Activity Measurement 3.1-6.3 µg/mL

Target

Target Organism S. typhimurium LT2 and 1102

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Source Activity Label	Source Definition
Antiviral	DRAMP antiviral activity supported by activity records against viral targets. dbAMP 3.0 classified under the Anti-viral function class.

Evidence Text

Active against Gram- S. typhimurium LT2 and 1102 (MIC 3.1-6.3 ug/ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S.

Evidence 17 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiviral

Activity Measurement 12.5 µg/mL

Target

Target Organism Escherichia coli K12

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Evidence Text

Active against Gram- S. typhimurium LT2 and 1102 (MIC 3.1-6.3 ug/ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC

Evidence 18 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiviral

Activity Measurement 3.1-12.5 µg/mL

Target

Target Organism S. Minnesota 1114W or R595

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Evidence Text

Evidence 19 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiviral

Activity Measurement 6.3-12.5 µg/mL

Target

Target Organism Staphylococcus aureus 209P or ATCC 25923

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Evidence Text

ml), E. coli K12 (MIC 12.5 ug/ml), S. Minnesota 1114W or R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC 6.3-12.5 ug/ml), and C. albicans M9 (MIC 6.3 ug/ml).

Evidence 20 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antiviral

Activity Measurement 6.3 µg/mL

Target

Target Organism Candida albicans M9

Source & Reference

Database Source dbAMP 3.0 DRAMP

Other

Evidence Text

r R595 (MIC 3.1-12.5 ug/ml), Gram+ S. aureus 209P or ATCC 25923 (MIC 6.3-12.5 ug/ml), and C. albicans M9 (MIC 6.3 ug/ml).

Evidence 21 Activity

Activity

Activity Endpoint 30% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 100 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

30% Hemolysis | 100 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III | Mar Drugs | 2018

Evidence 22 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 11.3±1.6 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 11.3±1.6 | µM | target cell: Human embryonic kidney HEK293T cells

Target

Target Cell Human embryonic kidney HEK293T cells

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III | Mar Drugs | 2018

Evidence 23 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 9.7±0.2 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 9.7±0.2 | µM | target cell: Human embryonic fibroblasts

Target

Target Cell Human embryonic fibroblasts

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III | Mar Drugs | 2018

Evidence 24 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 18.3±1.4 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 18.3±1.4 | µM | target cell: Human normal astrocytes

Target

Target Cell Human normal astrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Cytotoxic Potential of the Novel Horseshoe Crab Peptide Polyphemusin III | Mar Drugs | 2018

Evidence 25 Activity

Activity

Activity Endpoint 50% Cell death

Detail Source Activity Toxicity

Activity Measurement 13.2±3.2 µM

Toxicity Type Cytotoxic

Toxicity Raw

50% Cell death | 13.2±3.2 | µM | target cell: Human PBMC

Target

Target Cell Human PBMC

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Comparison of Cytotoxic Activity in Leukemic Lineages Reveals Important Features of ?-Hairpin Antimicrobial Peptides. | J Cell Biochem | 2017