TPDB

3D PDB MODEL

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TPDB ID TPDB05136

Activity

Antibacterial Anticancer Antifungal Antiparasitic Antiviral Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 iACP-DRLF Antifungipept CAFPdb AI4AVP dbAMP 3.0 HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 27-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

ALWMTLLKKVLKAAAKALNAVLVGANA

FASTA

>TPDB05136|Antibacterial;Anticancer;Antifungal;Antiparasitic;Antiviral;Hemolysis;Toxicity|length=27
ALWMTLLKKVLKAAAKALNAVLVGANA

Formula C129H224N34O31S1

Absent Amino Acids RDCEQHIFPSY

Common Amino Acids A

Mass 2779.47

PI 11.00

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 19

Net Charge +4

Boman Index -1.64

Hydrophobicity 1.004

Aliphatic Index 148.52

Half Life

Mammalian: 4.4 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 5500

Absorbance 280nm 197.88

Polar Residues 3

number

8

A

0

R

2

N

0

D

0

C

0

E

0

Q

1

G

0

H

0

I

6

L

4

K

1

M

0

F

0

P

0

S

1

T

1

W

0

Y

3

V

Amino Acid Distribution

A: 8 R: 0 N: 2 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 0 L: 6 K: 4 M: 1 F: 0 P: 0 S: 0 T: 1 W: 1 Y: 0 V: 3

Length

27

Formula

C129H224N34O31S1

Mass

2779.47

PI

11.00

Net Charge

+4

Boman Index

-1.64

Hydrophobicity

1.004

Evidence 1 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Hemolysis

Activity Measurement 20 µM

Hemolysis Raw

[Ref:11850249]IC50=20 µM against human erythrocytes

Target

RBC Target human erythrocytes

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Eur J Biochem. 1994 Jan 15 219(1-2):145-154.##Antimicrob Agents Chemother. 2002 Mar 46(3):689-694.##J Biol Chem. 1997 Dec 12 272(50):31609-31616.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 100% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 1 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

100% Hemolysis | 1 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

The vertebrate peptide antibiotics dermaseptins have overlapping structural features but target specific microorganisms. | J Biol Chem | 1994

Evidence 3 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 20 µM

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref:11850249]IC50=20 µM against human erythrocytes

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Eur J Biochem. 1994 Jan 15 219(1-2):145-154.##Antimicrob Agents Chemother. 2002 Mar 46(3):689-694.##J Biol Chem. 1997 Dec 12 272(50):31609-31616.

Evidence 4 Hemolysis

Activity

Activity Measurement 20 µM

Activity Endpoint IC50

Hemolysis Raw

[Ref:11850249]IC50=20 µM against human erythrocytes

Detail Source Activity Hemolysis

Target

RBC Target human erythrocytes

Source & Reference

source HemoPI PD_Hemolysis

Reference

Eur J Biochem. 1994 Jan 15 219(1-2):145-154.##Antimicrob Agents Chemother. 2002 Mar 46(3):689-694.##J Biol Chem. 1997 Dec 12 272(50):31609-31616.

Source DRAMP4

Database Source PD_Hemolysis