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Peptide record

TPDB04618

NZ2114 Antibacterial Cell-penetrating Peptides Toxicity standard
40 amino acids
Basic Information
3D PDB MODEL
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TPDB04618
NZ2114
Antibacterial Cell-penetrating Peptides Toxicity
Anti-infective peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 Peptipedia CPPsite3.0
standard
No
A 40-aa standard natural multi-activity (Antibacterial, Cell-penetrating Peptides, and Toxicity) peptide sequence curated from AntiBP3, Peptipedia, and CPPsite3.0, with an available 3D structural model.
Sequence
GFGCNGPWNEDDLRCHNHCKSIKGYKGGYCAKGGFVCKCY
Physicochemical Analysis
C191H280N56O54S6
QMT
G
4417.03
8.30
8
3
10
+3
4.74
-0.672
29.25
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
10345
234.21
13
Residue Composition
number
1
A
1
R
3
N
2
D
6
C
1
E
0
Q
8
G
2
H
1
I
1
L
5
K
0
M
2
F
1
P
1
S
0
T
1
W
3
Y
1
V
Amino Acid Distribution
A: 1 R: 1 N: 3 D: 2 C: 6 E: 1 Q: 0 G: 8 H: 2 I: 1 L: 1 K: 5 M: 0 F: 2 P: 1 S: 1 T: 0 W: 1 Y: 3 V: 1
Chemical Descriptors
40
C191H280N56O54S6
4417.03
8.30
+3
4.74
-0.672
Free
L
Linear
Free
NCC(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CS)C(=O)N[C@@H](CC(=O)N)C(=O)NCC(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CS)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1ccccc1)C(=O)O NCC(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](CS)C(=O)N[C@@H](CC(=O)N)C(=O)NCC(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=C(C(=NC1)C)CC)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC1=NC=NC1)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CS)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1ccccc1)C(=O)O
Evidence Records 5 records
Evidence 1 Activity

Activity

MIC
Antibacterial
0.028-0.114 µM

Target

Staphylococcus aureus ATCC 25923 or ATCC 6538 or MRSA ATCC 43300

Source & Reference

NA

Other

active against Gram+ S. aureus ATCC 25923 or ATCC 6538 or MRSA ATCC 43300 (MIC 0.028-0.114 uM), S. suis CVCC3309 or CVCC3928 or CVCC606 (MIC 0.028 uM), S. pneumoniae CVCC1.8722 or CVCC
Evidence 2 Activity

Activity

MIC
Antibacterial
0.028 µM

Target

S. suis CVCC3309 or CVCC3928 or CVCC606

Source & Reference

NA

Other

against Gram+ S. aureus ATCC 25923 or ATCC 6538 or MRSA ATCC 43300 (MIC 0.028-0.114 uM), S. suis CVCC3309 or CVCC3928 or CVCC606 (MIC 0.028 uM), S. pneumoniae CVCC1.8722 or CVCC2350 (MIC 0.454-0.909 uM), Gram- S-enteritidis CMCC50336
Evidence 3 Activity

Activity

Cell-penetrating Peptides

Target

RAW 264.7 Macrophages

Assay & Model

Six-week-old specifc pathogen-free female ICR mice
Six-week-old specifc pathogen-free female ICR mice

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L
Protein derived
FITC-labeled
Cytoplasm
The percentages of PI-staining in cells treated with NZ2114 were 5.34% at 250 μg/ml, respectively, suggesting that NZ2114 was more penetrable than MP1102.
Macropinocytosis and clathrin mediated endocytosis
In vitro and in vivo
CCSCCSSCCCCCSCSSSSSSCCCCCCTHHHHCSCCCCC
Evidence 4 Activity

Activity

Cell-penetrating Peptides

Target

Mac-T Cells (Infected With Mrsa And S. Aureus Cvcc3051)

Assay & Model

ICR mouse mastitis model
ICR mouse mastitis model

Source & Reference

CPPsite3.0
CPPsite3

Other

Natural residues Linear L
Protein derived
FITC-conjugated
Cytoplasm
Percentages of cells showing fluorescence in the case of NZ2114 is in range from 90.5–99.9%.
Clathrin mediated endocytosis
In vitro and in vivo
CCSSCSSSCCCCCTTCCSEETTEECCSCCSSSSCCCCCC
Evidence 5 Activity

Activity

0% Hemolysis
Toxicity
128 µg/mL
Hemolytic Cytotoxic
0% Hemolysis | 128 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
High expression of a plectasin-derived peptide NZ2114 in Pichia pastoris and its pharmacodynamics, postantibiotic and synergy against Staphylococcus aureus. | Appl Microbiol Biotechnol | 2014
Additional Detail Fields 1 fields
CCSCCSSCCCCCSCSSSSSSCCCCCCTHHHHCSCCCCC CCSSCSSSCCCCCTTCCSEETTEECCSCCSSSSCCCCCC