TPDB

3D PDB MODEL

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TPDB ID TPDB04433

Activity

Antibacterial Antifungal Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia CAFPdb PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 25-aa standard natural multi-activity (Antibacterial, Antifungal, Hemolysis, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

FIHHIIGGLFSVGKHIHSLIHRRRR

FASTA

>TPDB04433|Antibacterial;Antifungal;Hemolysis;Toxicity|length=25
FIHHIIGGLFSVGKHIHSLIHRRRR

Formula C137H220N48O28

Absent Amino Acids ANDCEQMPTWY

Common Amino Acids HI

Mass 2987.56

PI 12.88

Basic Residues 10

Acidic Residues 0

Hydrophobic Residues 10

Net Charge +5

Boman Index 4.46

Hydrophobicity -0.032

Aliphatic Index 120.80

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 0

Absorbance 280nm 0.00

Polar Residues 2

number

0

A

4

R

0

N

0

D

0

C

0

E

0

Q

3

G

5

H

5

I

2

L

1

K

0

M

2

F

0

P

2

S

0

T

0

W

0

Y

1

V

Amino Acid Distribution

A: 0 R: 4 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 3 H: 5 I: 5 L: 2 K: 1 M: 0 F: 2 P: 0 S: 2 T: 0 W: 0 Y: 0 V: 1

Length

25

Formula

C137H220N48O28

Mass

2987.56

PI

12.88

Net Charge

+5

Boman Index

4.46

Hydrophobicity

-0.032

Evidence 1 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Hemolysis

Activity Measurement 5 5 10 35 55 85 %

Hemolysis Raw

[Ref.29040295] 5% haemolysis at 1.56 µg/ml, 5% haemolysis at 3.13 µg/ml, 10% haemolysis at 6.25 µg/ml, 35% haemolysis at 12.50 µg/ml, 55% haemolysis at 25.00 µg/ml, 85% haemolysis at 50.00 µg/ml against mouse red blood cells

Target

RBC Target mouse red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

PLoS One. 2017 Oct 17 12(10):e0186442.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 18.75 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 18.75 | µg/mL | target cell: Mouse erythrocytes

Target

Target Cell Mouse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Hydrophobic residues are critical for the helix-forming, hemolytic and bactericidal activities of amphipathic antimicrobial peptide TP4. | PLoS One | 2017

Evidence 3 Activity

Activity

Activity Endpoint % Hemolysis

Detail Source Activity Toxicity

Activity Measurement 5 %

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.29040295] 5% haemolysis at 1.56 µg/ml, 5% haemolysis at 3.13 µg/ml, 10% haemolysis at 6.25 µg/ml, 35% haemolysis at 12.50 µg/ml, 55% haemolysis at 25.00 µg/ml, 85% haemolysis at 50.00 µg/ml against mouse red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

PLoS One. 2017 Oct 17 12(10):e0186442.

Evidence 4 Hemolysis

Activity

Activity Measurement 5 5 10 35 55 85 %

Activity Endpoint % Hemolysis

Hemolysis Raw

[Ref.29040295] 5% haemolysis at 1.56 µg/ml, 5% haemolysis at 3.13 µg/ml, 10% haemolysis at 6.25 µg/ml, 35% haemolysis at 12.50 µg/ml, 55% haemolysis at 25.00 µg/ml, 85% haemolysis at 50.00 µg/ml against mouse red blood cells

Detail Source Activity Hemolysis

Target

RBC Target mouse red blood cells

Source & Reference

source PD_Hemolysis

Reference

PLoS One. 2017 Oct 17 12(10):e0186442.

Source DRAMP4

Database Source PD_Hemolysis