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Peptide record

TPDB04304

OT20 Antibacterial Cell-penetrating Peptides Toxicity standard
20 amino acids
Basic Information
3D PDB MODEL
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TPDB04304
OT20
Antibacterial Cell-penetrating Peptides Toxicity
Anti-infective peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides
AntiBP3 Peptipedia CPPsite3.0
standard
No
A 20-aa standard natural multi-activity (Antibacterial, Cell-penetrating Peptides, and Toxicity) peptide sequence curated from AntiBP3, Peptipedia, and CPPsite3.0, with an available 3D structural model.
Sequence
TKPKGTKPKGTKPKGTKPKG
Physicochemical Analysis
C92H166N28O25
ARNDCEQHILMFSWYV
K
2064.50
11.36
8
0
0
+8
10.32
-2.100
0.00
Mammalian: 7.2 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
4
Residue Composition
number
0
A
0
R
0
N
0
D
0
C
0
E
0
Q
4
G
0
H
0
I
0
L
8
K
0
M
0
F
4
P
0
S
4
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 4 H: 0 I: 0 L: 0 K: 8 M: 0 F: 0 P: 4 S: 0 T: 4 W: 0 Y: 0 V: 0
Chemical Descriptors
20
C92H166N28O25
2064.50
11.36
+8
10.32
-2.100
Free
L
Cationic
Linear
Free
N[C@@H](CCCN=C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCCN=C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=C(C)/C/1=C\C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O
Evidence Records 6 records
Evidence 1 Activity

Activity

Cell-penetrating Peptides

Target

Monomac6 Human Monocytes

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Protein derived
No difference in cellular uptake slightly between Cf-labelled and non-labelled
In vitro
CCCSSSTTCCCTTTTC
Evidence 2 Activity

Activity

50% Hemolysis
Toxicity
>300 µM
Hemolytic Cytotoxic
50% Hemolysis | >300 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Comparative analysis of internalisation, haemolytic, cytotoxic and antibacterial effect of membrane-active cationic peptides: aspects of experimental setup. | Amino Acids | 2017
Evidence 3 Activity

Activity

IC50
Toxicity
>300 µM
Cytotoxic
IC50 | >300 | µM | target cell: Human PBMC

Target

Human PBMC

Source & Reference

NA
DBAASP
Comparative analysis of internalisation, haemolytic, cytotoxic and antibacterial effect of membrane-active cationic peptides: aspects of experimental setup. | Amino Acids | 2017
Evidence 4 Activity

Activity

(91.7±3.56) Viability
Toxicity
1.5 µg/mL
Cytotoxic
(91.7±3.56) Viability | 1.5 | µg/mL | target cell: Mouse splenocytes

Target

Mouse splenocytes

Source & Reference

NA
DBAASP
Synthesis, conformation, and immunoreactivity of new carrier molecules based on repeated tuftsin-like sequence. | Biopolymers | 2004
Evidence 5 Activity

Activity

(93.7±2.04) Viability
Toxicity
15 µg/mL
Cytotoxic
(93.7±2.04) Viability | 15 | µg/mL | target cell: Mouse splenocytes

Target

Mouse splenocytes

Source & Reference

NA
DBAASP
Synthesis, conformation, and immunoreactivity of new carrier molecules based on repeated tuftsin-like sequence. | Biopolymers | 2004
Evidence 6 Activity

Activity

(92.2±1.83) Viability
Toxicity
50 µg/mL
Cytotoxic
(92.2±1.83) Viability | 50 | µg/mL | target cell: Mouse splenocytes

Target

Mouse splenocytes

Source & Reference

NA
DBAASP
Synthesis, conformation, and immunoreactivity of new carrier molecules based on repeated tuftsin-like sequence. | Biopolymers | 2004
Additional Detail Fields 1 fields
CCCSSSTTCCCTTTTC