TPDB

3D PDB MODEL

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TPDB ID TPDB03669

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 11-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from AntiBP3, Peptipedia, HemoPI, and other sources, with an available 3D structural model.

Sequence

LKLFKKILKYL

FASTA

>TPDB03669|Antibacterial;Anticancer;Hemolysis;Toxicity|length=11
LKLFKKILKYL

Formula C72H123N15O13

Absent Amino Acids ARNDCEQGHMPSTWV

Common Amino Acids LK

Mass 1406.86

PI 10.64

Basic Residues 4

Acidic Residues 0

Hydrophobic Residues 7

Net Charge +4

Boman Index -0.66

Hydrophobicity 0.509

Aliphatic Index 177.27

Half Life

Mammalian: 5.5 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 105.91

Polar Residues 1

number

0

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

1

I

4

L

4

K

0

M

1

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 1 L: 4 K: 4 M: 0 F: 1 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

11

Formula

C72H123N15O13

Mass

1406.86

PI

10.64

Net Charge

+4

Boman Index

-0.66

Hydrophobicity

0.509

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 5.0-7.5 µM

Target

Target Organism v. syringae

Source & Reference

Database Source NA

Other

Evidence Text

active against plant pathogenic bacterial strains P. syringae pv. syringae (MIC 5.0-7.5 uM), X. axonopodis pv. vesicatoria (MIC 2.5-5.0 uM), and E. amylovora (MIC 5.0-7.5 uM).

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 2.5-5.0 µM

Target

Target Organism v. vesicatoria

Source & Reference

Database Source NA

Other

Evidence Text

nt pathogenic bacterial strains P. syringae pv. syringae (MIC 5.0-7.5 uM), X. axonopodis pv. vesicatoria (MIC 2.5-5.0 uM), and E. amylovora (MIC 5.0-7.5 uM).

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 5.0-7.5 µM

Target

Target Organism E. amylovora

Source & Reference

Database Source NA

Other

Evidence Text

ringae pv. syringae (MIC 5.0-7.5 uM), X. axonopodis pv. vesicatoria (MIC 2.5-5.0 uM), and E. amylovora (MIC 5.0-7.5 uM).

Evidence 4 Activity

Activity

Activity Endpoint (91±6.2)% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 150 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

(91±6.2)% Hemolysis | 150 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Evidence 5 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Toxicity

Activity Measurement 39.0±8.5 µM

Toxicity Type Cytotoxic

Toxicity Raw

IC50 | 39.0±8.5 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Target Cell Mouse fibroblasts NIH 3T3

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Identification of BP16 as a non-toxic cell-penetrating peptide with highly efficient drug delivery properties. | Org Biomol Chem | 2014

Evidence 6 Hemolysis

Source & Reference

source HemoPI PD_Hemolysis

Database Source PD_Hemolysis