TPDB

3D PDB MODEL

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TPDB ID TPDB03378

Activity

Antibacterial Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 26-aa standard natural multi-activity (Antibacterial, Hemolysis, and Toxicity) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

KWKSFLKTFKSLKKTVLHTALKLISS

FASTA

>TPDB03378|Antibacterial;Hemolysis;Toxicity|length=26
KWKSFLKTFKSLKKTVLHTALKLISS

Formula C145H242N36O34

Absent Amino Acids RNDCEQGMPY

Common Amino Acids K

Mass 3033.74

PI 11.29

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +7

Boman Index 1.51

Hydrophobicity -0.062

Aliphatic Index 105.00

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 181.29

Polar Residues 7

number

1

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

1

H

1

I

5

L

7

K

0

M

2

F

0

P

4

S

3

T

1

W

0

Y

1

V

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 1 I: 1 L: 5 K: 7 M: 0 F: 2 P: 0 S: 4 T: 3 W: 1 Y: 0 V: 1

Length

26

Formula

C145H242N36O34

Mass

3033.74

PI

11.29

Net Charge

+7

Boman Index

1.51

Hydrophobicity

-0.062

Evidence 1 Activity

Activity

Activity Endpoint MHC

Detail Source Activity Hemolysis

Activity Measurement 15.6 µg/mL

Hemolysis Raw

[Ref.17158938]MHC=15.6 µg/ml against human red blood cells

Target

RBC Target human red blood cells

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Antimicrob Agents Chemother. 2007 Apr 51(4):1398-406.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 0% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 15.6 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

0% Hemolysis | 15.6 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Role of peptide hydrophobicity in the mechanism of action of alpha-helical antimicrobial peptides | Antimicrob Agents Chemother | 2007

Evidence 3 Activity

Activity

Activity Endpoint MHC

Detail Source Activity Toxicity

Activity Measurement 15.6 µg/mL

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.17158938]MHC=15.6 µg/ml against human red blood cells

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Antimicrob Agents Chemother. 2007 Apr 51(4):1398-406.

Evidence 4 Hemolysis

Activity

Activity Measurement 15.6 µg/mL

Activity Endpoint MHC

Hemolysis Raw

[Ref.17158938]MHC=15.6 µg/ml against human red blood cells

Detail Source Activity Hemolysis

Target

RBC Target human red blood cells

Source & Reference

source HemoPI PD_Hemolysis

Reference

Antimicrob Agents Chemother. 2007 Apr 51(4):1398-406.

Source DRAMP4

Database Source PD_Hemolysis