TPDB

3D PDB MODEL

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TPDB ID TPDB03185

Activity

Antibacterial Anticancer Antifungal Antiparasitic Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 20-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

KWKSFIKKLLSKFLHSAKKF

FASTA

>TPDB03185|Antibacterial;Anticancer;Antifungal;Antiparasitic;Hemolysis;Toxicity|length=20
KWKSFIKKLLSKFLHSAKKF

Formula C122H194N30O24

Absent Amino Acids RNDCEQGMPTYV

Common Amino Acids K

Mass 2465.07

PI 11.29

Basic Residues 8

Acidic Residues 0

Hydrophobic Residues 9

Net Charge +7

Boman Index 0.88

Hydrophobicity -0.385

Aliphatic Index 83.00

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 5500

Absorbance 280nm 223.12

Polar Residues 3

number

1

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

1

H

1

I

3

L

7

K

0

M

3

F

0

P

3

S

0

T

1

W

0

Y

0

V

Amino Acid Distribution

A: 1 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 1 I: 1 L: 3 K: 7 M: 0 F: 3 P: 0 S: 3 T: 0 W: 1 Y: 0 V: 0

Length

20

Formula

C122H194N30O24

Mass

2465.07

PI

11.29

Net Charge

+7

Boman Index

0.88

Hydrophobicity

-0.385

Evidence 1 Activity

Activity

Activity Endpoint MHC

Detail Source Activity Hemolysis

Activity Measurement 125 µg/mL

Hemolysis Raw

[Ref.22518150]MHC<125 µg/mL against human erythrocytes

Target

RBC Target human erythrocytes

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Int J Microbiol. 2012 2012:764834.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 0% Hemolysis

Detail Source Activity Toxicity

Activity Measurement <125 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

0% Hemolysis | <125 | µg/mL | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

The Design and Construction of K11: A Novel ?-Helical Antimicrobial Peptide | Int J Microbiol | 2012

Evidence 3 Activity

Activity

Activity Endpoint MHC

Detail Source Activity Toxicity

Activity Measurement 125 µg/mL

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.22518150]MHC<125 µg/mL against human erythrocytes

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Int J Microbiol. 2012 2012:764834.

Evidence 4 Hemolysis

Activity

Activity Measurement 125 µg/mL

Activity Endpoint MHC

Hemolysis Raw

[Ref.22518150]MHC<125 µg/mL against human erythrocytes

Detail Source Activity Hemolysis

Target

RBC Target human erythrocytes

Source & Reference

source HemoPI PD_Hemolysis

Reference

Int J Microbiol. 2012 2012:764834.

Source DRAMP4

Database Source PD_Hemolysis