TPDB

3D PDB MODEL

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TPDB ID TPDB03115

Activity

Antibacterial Anticancer Hemolysis Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides

Database_Source

AntiBP3 dbAMP DRAMP Peptipedia HemoPI Hemolytic2 PD_Hemolysis

Sequence_Type

standard

Has_Modification No

Description A 19-aa standard natural multi-activity (Antibacterial, Anticancer, Hemolysis, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.

Sequence

CWKWKWKWGSGWKWKWKWC

FASTA

>TPDB03115|Antibacterial;Anticancer;Hemolysis;Toxicity|length=19
CWKWKWKWGSGWKWKWKWC

Formula C137H175N33O21S2

Absent Amino Acids ARNDEQHILMFPTYV

Common Amino Acids W

Mass 2684.23

PI 10.54

Basic Residues 6

Acidic Residues 0

Hydrophobic Residues 8

Net Charge +6

Boman Index -9.19

Hydrophobicity -1.432

Aliphatic Index 0.00

Half Life

Mammalian: 1.2 hour Yeast: >20 hour E.coli: >10 hour

Extinction Coefficient Cystines 44125

Absorbance 280nm 1643.86

Polar Residues 3

number

0

A

0

R

0

N

0

D

2

C

0

E

0

Q

2

G

0

H

0

I

0

L

6

K

0

M

0

F

0

P

1

S

0

T

8

W

0

Y

0

V

Amino Acid Distribution

A: 0 R: 0 N: 0 D: 0 C: 2 E: 0 Q: 0 G: 2 H: 0 I: 0 L: 0 K: 6 M: 0 F: 0 P: 0 S: 1 T: 0 W: 8 Y: 0 V: 0

Length

19

Formula

C137H175N33O21S2

Mass

2684.23

PI

10.54

Net Charge

+6

Boman Index

-9.19

Hydrophobicity

-1.432

Evidence 1 Activity

Activity

Activity Endpoint EC50

Detail Source Activity Hemolysis

Activity Measurement 45000 nM

Hemolysis Raw

[Ref.15328096]EC50=45000 nM against human erythrocytes

Target

RBC Target human erythrocytes

Source & Reference

Database Source Hemolytik PD_Hemolysis

Source DRAMP4

Reference

Antimicrob Agents Chemother. 2004 Sep 48(9):3349-3357.

Other

Source Activity Label	Source Definition
Hemolysis	Hemolytik experimentally validated hemolytic peptide annotation.

Evidence 2 Activity

Activity

Activity Endpoint 50% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 45 µM

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

50% Hemolysis | 45 | µM | target cell: Human erythrocytes

Target

Target Cell Human erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

De novo design of potent antimicrobial peptides | Antimicrob Agents Chemother | 2004

Evidence 3 Activity

Activity

Activity Endpoint EC50

Detail Source Activity Toxicity

Activity Measurement 45000 nM

Toxicity Type Hemolytic

Toxicity Raw

Hemolytic_activity: [Ref.15328096]EC50=45000 nM against human erythrocytes

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Antimicrob Agents Chemother. 2004 Sep 48(9):3349-3357.

Evidence 4 Hemolysis

Activity

Activity Measurement 45000 nM

Activity Endpoint EC50

Hemolysis Raw

[Ref.15328096]EC50=45000 nM against human erythrocytes

Detail Source Activity Hemolysis

Target

RBC Target human erythrocytes

Source & Reference

source HemoPI PD_Hemolysis

Reference

Antimicrob Agents Chemother. 2004 Sep 48(9):3349-3357.

Source DRAMP4

Database Source PD_Hemolysis