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Peptide record

TPDB01303

Antioxidative peptide Antioxidant standard
7 amino acids
Basic Information
3D PDB MODEL
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TPDB01303
Antioxidative peptide
Antioxidant
Functional / health-promoting peptides
AnOxPePred - 1.0 AODB AoXpDb Multi-AOP pep-lab Peptipedia
standard
No
A 7-aa standard natural antioxidant peptide sequence curated from AnOxPePred - 1.0, AODB, AoXpDb, and other sources, with an available 3D structural model.
Sequence
NGLEGLK
Physicochemical Analysis
C31H55N9O11
ARDCQHIMFPSTWYV
GL
729.83
6.94
1
1
2
-0
2.33
-0.586
111.43
Mammalian: 1.4 hour Yeast: 3 min E.coli: >10 hour
0
0.00
1
Residue Composition
number
0
A
0
R
1
N
0
D
0
C
1
E
0
Q
2
G
0
H
0
I
2
L
1
K
0
M
0
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 0 R: 0 N: 1 D: 0 C: 0 E: 1 Q: 0 G: 2 H: 0 I: 0 L: 2 K: 1 M: 0 F: 0 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
7
C31H55N9O11
729.83
6.94
-0
2.33
-0.586
747 Da
6.92
0.00
N[C@@]([H])(CC(=O)N)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCCCN)C(=O)O
729.83 Da
Evidence Records 1 records
Evidence 1 Activity

Activity

Antioxidative activity
The peptide exhibited their antioxidant potential to act as chain-breaking antioxidants by inhibiting radical-mediated peroxidation of linoleic acid, and their activities were closer to highly active synthetic antioxidant, butylated hydroxytoluene after 7 days (95,8%). Electron spin trapping studies revealed that the peptides were potent scavengers of free radicals in the order of carbon-centered (IC 50 = 304,67 μM), hydroxyl (IC 50 = 428.54 μM) and superoxide radicals (IC 50 = 573.83 μM).
Literature report: According to the results of MTT assay, squid antioxidative peptides did not exhibit any cytotoxic effect to the lung fibroblasts at tested concentrations (0-100 μg/ml) and the peptide NGLEGLK significantly ( P < 0.05) enhanced the viability of t-BHP-induced cytotoxicity up to 83% at the concentration of 75 μg/ml. Prediction: ToxinPred
Antioxidant

Assay & Model

linoleic acid peroxidation superoxide radical scavenging
Enzymatic hydrolysis
Peptide obtained by hydrolysis of giant squid muscle protein by use of trypsin for 6 h.

Source & Reference

AODB AoXpDb
Rajapakse N, Mendis E, Byun HG, Kim SK. Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. J Nutr Biochem. 2005 Sep 16(9):562-9.
10.1016/j.jnutbio.2005.02.005
[1] Jung W K, Qian Z J, Lee S H, et al. Free radical scavenging activity of a novel antioxidative peptide isolated from in vitro gastrointestinal digests of Mytilus coruscus.[J]. Journal of Medicinal Food, 2007, 10(1):197-202.
2005
DFBP

Other

Native peptide
Animal
Giant squid (Dosidicus gigas)
Muscle protein