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Peptide record

TPDB01292

Antioxidative peptide Antioxidant standard
13 amino acids
Basic Information
3D PDB MODEL
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TPDB01292
Antioxidative peptide
Antioxidant
Functional / health-promoting peptides
AnOxPePred - 1.0 AODB AoXpDb Multi-AOP pep-lab Peptipedia
standard
No
A 13-aa standard natural antioxidant peptide sequence curated from AnOxPePred - 1.0, AODB, AoXpDb, and other sources, with an available 3D structural model.
Sequence
NADFGLNGLEGLA
Physicochemical Analysis
C56H87N15O20
RCQHIKMPSTWYV
GL
1290.40
3.01
0
2
6
-2
0.97
0.200
105.38
Mammalian: 1.4 hour Yeast: 3 min E.coli: >10 hour
0
0.00
2
Residue Composition
number
2
A
0
R
2
N
1
D
0
C
1
E
0
Q
3
G
0
H
0
I
3
L
0
K
0
M
1
F
0
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 2 R: 0 N: 2 D: 1 C: 0 E: 1 Q: 0 G: 3 H: 0 I: 0 L: 3 K: 0 M: 0 F: 1 P: 0 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
13
C56H87N15O20
1290.40
3.01
-2
0.97
0.200
1307 Da
3.04
0.00
N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C)C(=O)O
1290.38 Da
Evidence Records 1 records
Evidence 1 Activity

Activity

Antioxidative activity
The peptide exhibited their antioxidant potential to act as chain-breaking antioxidants by inhibiting radical-mediated peroxidation of linoleic acid, and their activities were closer to highly active synthetic antioxidant, butylated hydroxytolueneafter 7 days (87,7%). Electron spin trapping studies revealed that the peptides were potent scavengers of free radicals in the order of carbon-centered (IC 50 = 396.04 μM), hydroxyl (IC 50 = 497.32 μM) and superoxide radicals (IC 50 = 669.34 μM).
Literature report: According to the results of MTT assay, squid antioxidative peptides did not exhibit any cytotoxic effect to the lung fibroblasts at tested concentrations (0-100 μg/ml) and the peptide NADFGLNGLEGLA significantly ( P < 0.05) enhanced the viability of t-BHP-induced cytotoxicity up to 74% at the concentration of 75 μg/ml. Prediction: ToxinPred
Antioxidant

Assay & Model

linoleic acid peroxidation superoxide radical scavenging
Enzymatic hydrolysis
Peptide obtained by hydrolysis of giant squid muscle protein by use of trypsin for 6 h.

Source & Reference

AODB AoXpDb
Rajapakse N, Mendis E, Byun HG, Kim SK. Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. J Nutr Biochem. 2005 Sep 16(9):562-9.
10.1016/j.jnutbio.2005.02.005
[1] Jung W K, Qian Z J, Lee S H, et al. Free radical scavenging activity of a novel antioxidative peptide isolated from in vitro gastrointestinal digests of Mytilus coruscus.[J]. Journal of Medicinal Food, 2007, 10(1):197-202.
2005
DFBP

Other

Native peptide
Animal, Marine
Giant squid (Dosidicus gigas)
Muscle protein