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Peptide record

TPDB00928

Antibacterial Anticancer Antifungal Antioxidant Toxicity standard

34 amino acids

3D PDB MODEL

Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.

TPDB ID TPDB00928

Activity

Antibacterial Anticancer Antifungal Antioxidant Toxicity

Activity_category_statistics

Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Functional / health-promoting peptides

Database_Source

AntiBP3 Peptipedia CAFPdb dbAMP AODB ToxinPred 3.0

Sequence_Type

standard

Has_Modification No

Description A 34-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, Peptipedia, CAFPdb, and other sources, with an available 3D structural model.

Sequence

KKCNFFCKLKKKVKSVGSRNLIGSATHHHRIYRV

FASTA

>TPDB00928|Antibacterial;Anticancer;Antifungal;Antioxidant;Toxicity|length=34
KKCNFFCKLKKKVKSVGSRNLIGSATHHHRIYRV

Formula C178H296N58O42S2

Absent Amino Acids DEQMPW

Common Amino Acids K

Mass 3984.79

PI 11.23

Basic Residues 13

Acidic Residues 0

Hydrophobic Residues 11

Net Charge +10

Boman Index 8.25

Hydrophobicity -0.618

Aliphatic Index 74.41

Half Life

Mammalian: 1.3 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 1615

Absorbance 280nm 40.53

Polar Residues 9

number

Amino Acid Distribution

A: 1 R: 3 N: 2 D: 0 C: 2 E: 0 Q: 0 G: 2 H: 3 I: 2 L: 2 K: 7 M: 0 F: 2 P: 0 S: 3 T: 1 W: 0 Y: 1 V: 3

Length

Formula

C178H296N58O42S2

Mass

3984.79

11.23

Net Charge

+10

Boman Index

8.25

Hydrophobicity

-0.618

Evidence 1 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 9.4 µM

Target

Target Organism A. sobria

Source & Reference

Database Source dbAMP

Other

Source Activity Label	Source Definition
Antibacterial	dbAMP classified as Antibacterial under the Anti-bacterial function class.

Evidence Text

Active against Gram- E. coli CMCC44102, A. sobria (MIC 9.4 uM), E. coli ATCC 25922, K. pneumoniae, V. Harvey, V. splendidus , (MIC 2.4 uM), S. castellan

Evidence 2 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4.7 µM

Target

Target Organism V. parahaemolyticus

Source & Reference

Database Source dbAMP

Other

Evidence Text

. Harvey, V. splendidus , (MIC 2.4 uM), S. castellani ATCC12022, P. aeruginosa CMCC10104, V. parahaemolyticus (MIC 4.7 uM), (inactive) V.alginolyticu, V.vulnificus, A.hydrophila, A.baumnnii (MIC all >100 uM) Gra

Evidence 3 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 4.7 µM

Target

Target Organism Clostridium perfringens ATCC13124

Source & Reference

Database Source dbAMP

Other

Evidence Text

ulnificus, A.hydrophila, A.baumnnii (MIC all >100 uM) Gram+ active: S. aureus CMCC26003, C. perfringens ATCC13124 (MIC 4.7 uM), B. subtilis CMCC63501 (MIC 9.4 uM), inactive: B.cereus CMCC63301, E.faecalis ATCC29212,

Evidence 4 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement 9.4 µM

Target

Target Organism B. subtilis CMCC63501

Source & Reference

Database Source dbAMP

Other

Evidence Text

C all >100 uM) Gram+ active: S. aureus CMCC26003, C. perfringens ATCC13124 (MIC 4.7 uM), B. subtilis CMCC63501 (MIC 9.4 uM), inactive: B.cereus CMCC63301, E.faecalis ATCC29212, S.epidermidis, E.faecium (MIC >100 u

Evidence 5 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >100 µM

Target

Target Organism E.faecium

Source & Reference

Database Source dbAMP

Other

Evidence Text

MCC63501 (MIC 9.4 uM), inactive: B.cereus CMCC63301, E.faecalis ATCC29212, S.epidermidis, E.faecium (MIC >100 uM), fungi C.albicans and C.glabrata (MIC >100 uM). Reg. 7/2021

Evidence 6 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antibacterial

Activity Measurement >100 µM

Target

Target Organism C.glabrata

Source & Reference

Database Source dbAMP

Other

Evidence Text

63301, E.faecalis ATCC29212, S.epidermidis, E.faecium (MIC >100 uM), fungi C.albicans and C.glabrata (MIC >100 uM). Reg. 7/2021

Evidence 7 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 9.4 µM

Target

Target Organism A. sobria

Source & Reference

Database Source dbAMP CAFPdb

Other

Source Activity Label	Source Definition
Antifungal	dbAMP classified under the Anti-fungal function class.

Evidence Text

Active against Gram- E. coli CMCC44102, A. sobria (MIC 9.4 uM), E. coli ATCC 25922, K. pneumoniae, V. Harvey, V. splendidus , (MIC 2.4 uM), S. castellan

Evidence 8 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 4.7 µM

Target

Target Organism V. parahaemolyticus

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

Evidence 9 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 4.7 µM

Target

Target Organism Clostridium perfringens ATCC13124

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

Evidence 10 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement 9.4 µM

Target

Target Organism B. subtilis CMCC63501

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

Evidence 11 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement >100 µM

Target

Target Organism E.faecium

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

MCC63501 (MIC 9.4 uM), inactive: B.cereus CMCC63301, E.faecalis ATCC29212, S.epidermidis, E.faecium (MIC >100 uM), fungi C.albicans and C.glabrata (MIC >100 uM). Reg. 7/2021

Evidence 12 Activity

Activity

Activity Endpoint MIC

Detail Source Activity Antifungal

Activity Measurement >100 µM

Target

Target Organism C.glabrata

Source & Reference

Database Source dbAMP CAFPdb

Other

Evidence Text

63301, E.faecalis ATCC29212, S.epidermidis, E.faecium (MIC >100 uM), fungi C.albicans and C.glabrata (MIC >100 uM). Reg. 7/2021

Evidence 13 Activity

Activity

Activity Endpoint 5.9% Hemolysis

Detail Source Activity Toxicity

Activity Measurement 200 µg/mL

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

5.9% Hemolysis | 200 | µg/mL | target cell: Mouse erythrocytes

Target

Target Cell Mouse erythrocytes

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Identification and characterization of novel bi-functional cathelicidins from the black-spotted frog (Pelophylax nigromaculata) with both anti-infective and antioxidant activities. | Dev Comp Immunol | 2021

Evidence 14 Activity

Activity

Activity Endpoint 20.8% Killing

Detail Source Activity Toxicity

Activity Measurement 200 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

20.8% Killing | 200 | µg/mL | target cell: Human keratinocytes HaCat

Target

Target Cell Human keratinocytes HaCat

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Evidence 15 Activity

Activity

Activity Endpoint 46.3% Killing

Detail Source Activity Toxicity

Activity Measurement 200 µg/mL

Toxicity Type Cytotoxic

Toxicity Raw

46.3% Killing | 200 | µg/mL | target cell: Murine peritoneal macrophages

Target

Target Cell Murine peritoneal macrophages

Source & Reference

Database Source NA

Source Database DBAASP

Reference

Evidence 16 Activity

Activity

Detail Source Activity Toxicity

Toxicity Type Hemolytic Cytotoxic

Toxicity Raw

Hemolytic_activity: Not available | Cytotoxicity: Not available

Target

Target Cell erythrocytes

Source & Reference

Database Source NA

Source Database DRAMP

Reference

Dev Comp Immunol. 2021 Mar 116:103928.