TPDB

3D PDB MODEL

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TPDB ID TPDB00791

Peptide_Name

ACE inhibitor Antihypertensive peptide derived from spinach Rubisco

Activity

ACE inhibitors Antihypertensive Antioxidant

Activity_category_statistics

Cardiometabolic peptides Functional / health-promoting peptides

Database_Source

pep-lab Peptipedia AHTPDB AHTpin

Sequence_Type

standard

Has_Modification No

Description A 7-aa standard natural multi-activity (ACE inhibitors, Antihypertensive, and Antioxidant) peptide sequence curated from pep-lab, Peptipedia, AHTPDB, and other sources, with an available 3D structural model.

Sequence

IAYKPAG

FASTA

>TPDB00791|ACE inhibitors;Antihypertensive;Antioxidant|length=7
IAYKPAG

Formula C34H54N8O9

Absent Amino Acids RNDCEQHLMFSTWV

Common Amino Acids A

Mass 718.85

PI 9.74

Basic Residues 1

Acidic Residues 0

Hydrophobic Residues 4

Net Charge +1

Boman Index -0.14

Hydrophobicity 0.129

Aliphatic Index 84.29

Half Life

Mammalian: 20 hour Yeast: 30 min E.coli: >10 hour

Extinction Coefficient Cystines 1490

Absorbance 280nm 207.28

Polar Residues 1

number

2

A

0

R

0

N

0

D

0

C

0

E

0

Q

1

G

0

H

1

I

0

L

1

K

0

M

0

F

1

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 2 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 1 H: 0 I: 1 L: 0 K: 1 M: 0 F: 0 P: 1 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

7

Formula

C34H54N8O9

Mass

718.85

PI

9.74

Net Charge

+1

Boman Index

-0.14

Hydrophobicity

0.129

Chemical_Mass

718.8386

Monoisotopic_Mass

718.4001

Evidence 1 Activity

Activity

Activity Endpoint IC50

Detail Source Activity ACE inhibitors

Activity Measurement 4.20 µM

Function Inhibitor of Angiotensin-converting enzyme (ACE) (EC 3.4.15.1 MEROPS ID: M02-001)

Detail Activity ACE inhibitor

Activity Code ah

Source & Reference

Database Source NA

Authors Yang Y., Marczak E.D., Yokoo M., Usui H., Yoshikawa M.

Title

Isolation and antihypertensive effect of angiotensin I-converting enzyme (ACE) inhibitory peptides from spinach Rubisco. J. Agric. Food. Chem. 51(17): 4897-4902, 2003

Year 2003

Literature Source Journal

Other

Number of Residues 7

Additional Info

BIOPEP-UWM database of bioactive peptides SMILES: N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C)C(=O)NCC(=O)O InChI=1S/C34H54N8O9/c1-5-19(2)28(36)33(50)39-21(4)30(47)41-25(17-22-11-13-23(43)14-12-22)31(48)40-24(9-6-7-15-35)34(51)42-16-8-10-26(42)32(49)38-20(3)29(46)37-18-27(44)45/h11-14,19-21,24-26,28,43H,5-10,15-18,35-36H2,1-4H3,(H,37,46)(H,38,49)(H,39,50)(H,40,48)(H,41,47)(H,44,45)/t19-,20-,21-,24-,25-,26-,28-/m0/s1 InChIKey=GPOHMCMFILTZSA-NTHYOZQESA-N Inhibitor of Angiotensin-converting enzyme (ACE) (EC 3.4.15.1 MEROPS ID: M02-001) according to the AHTPDB database the EROP-Moscow database

Evidence 2 Activity

Activity

Activity Endpoint EC50

Detail Source Activity Antihypertensive

Activity Measurement 0.00 µM

Function Antihypertensive effect after oral administration to spontaneously hypertensive rats

Detail Activity hypotensive

Activity Code hyp

Source & Reference

Database Source AHTPDB

Authors Yang Y., Marczak E.D., Yokoo M., Usui H., Yoshikawa M.

Title

Isolation and antihypertensive effect of angiotensin I-converting enzyme (ACE) inhibitory peptides from spinach Rubisco. J. Agric. Food. Chem. 51(17): 4897-4902, 2003

Year 2003

Literature Source Journal

Other

Source Activity Label	Source Definition
Antihypertensive	AHTPDB manually curated experimentally validated antihypertensive peptide annotation.

Number of Residues 7

Additional Info

BIOPEP-UWM database of bioactive peptides SMILES: N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C)C(=O)NCC(=O)O InChI=1S/C34H54N8O9/c1-5-19(2)28(36)33(50)39-21(4)30(47)41-25(17-22-11-13-23(43)14-12-22)31(48)40-24(9-6-7-15-35)34(51)42-16-8-10-26(42)32(49)38-20(3)29(46)37-18-27(44)45/h11-14,19-21,24-26,28,43H,5-10,15-18,35-36H2,1-4H3,(H,37,46)(H,38,49)(H,39,50)(H,40,48)(H,41,47)(H,44,45)/t19-,20-,21-,24-,25-,26-,28-/m0/s1 InChIKey=GPOHMCMFILTZSA-NTHYOZQESA-N Inhibitor of Angiotensin-converting enzyme (ACE) (EC 3.4.15.1 MEROPS ID: M02-001) according to the AHTPDB database the BIOPEP-UWM database of bioactive peptides (ID 9665) the EROP-Moscow database

Evidence 3 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Antihypertensive

Activity Measurement 4.20 µM

Function Inhibitor of Angiotensin-converting enzyme (ACE) (EC 3.4.15.1 MEROPS ID: M02-001)

Detail Activity ACE inhibitor

Activity Code ah

Source & Reference

Database Source AHTPDB

Authors Yang Y., Marczak E.D., Yokoo M., Usui H., Yoshikawa M.

Title

Isolation and antihypertensive effect of angiotensin I-converting enzyme (ACE) inhibitory peptides from spinach Rubisco. J. Agric. Food. Chem. 51(17): 4897-4902, 2003

Year 2003

Literature Source Journal

Other

Number of Residues 7

Additional Info

BIOPEP-UWM database of bioactive peptides SMILES: N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C)C(=O)NCC(=O)O InChI=1S/C34H54N8O9/c1-5-19(2)28(36)33(50)39-21(4)30(47)41-25(17-22-11-13-23(43)14-12-22)31(48)40-24(9-6-7-15-35)34(51)42-16-8-10-26(42)32(49)38-20(3)29(46)37-18-27(44)45/h11-14,19-21,24-26,28,43H,5-10,15-18,35-36H2,1-4H3,(H,37,46)(H,38,49)(H,39,50)(H,40,48)(H,41,47)(H,44,45)/t19-,20-,21-,24-,25-,26-,28-/m0/s1 InChIKey=GPOHMCMFILTZSA-NTHYOZQESA-N Inhibitor of Angiotensin-converting enzyme (ACE) (EC 3.4.15.1 MEROPS ID: M02-001) according to the AHTPDB database the EROP-Moscow database

Number_of_Residues

7