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Peptide record

TPDB00615

Magainin 2 Magainin II Antibacterial Anticancer Antifungal Antioxidant Antiparasitic Antiviral Cell-penetrating Peptides Hemolysis Toxicity standard
23 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB00615
Magainin 2 Magainin II
Antibacterial Anticancer Antifungal Antioxidant Antiparasitic Antiviral Cell-penetrating Peptides Hemolysis Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides Functional / health-promoting peptides
AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 CancerPPD 2.0 SATPdb dbACP iACP-DRLF AFP-GFuse Antifungipept CAFPdb dbAMP 3.0 i2APP AI4AVP DRAVP 2.0 CPPsite3.0 APD_complete HemoPI Hemolytic2 PD_Hemolysis ToxinPred 3.0
standard
No
A 23-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.
Sequence
GIGKFLHSAKKFGKAFVGEIMNS
Physicochemical Analysis
C114H180N30O29S1
RDCQPTWY
GK
2466.93
10.55
5
1
10
+3
2.59
0.083
72.17
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
3
Residue Composition
number
2
A
0
R
1
N
0
D
0
C
1
E
0
Q
4
G
1
H
2
I
1
L
4
K
1
M
3
F
0
P
2
S
0
T
0
W
0
Y
1
V
Amino Acid Distribution
A: 2 R: 0 N: 1 D: 0 C: 0 E: 1 Q: 0 G: 4 H: 1 I: 2 L: 1 K: 4 M: 1 F: 3 P: 0 S: 2 T: 0 W: 0 Y: 0 V: 1
Chemical Descriptors
23
C114H180N30O29S1
2466.93
10.55
+3
2.59
0.083
Free
L
Cationic
Linear
Linear
Free
Antimicrobial
Xenopus laevis
N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CS)C(=O)N[C@@H](Cc1[nH]cnc1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CO)C(=O)NCC(=O)O
Evidence Records 69 records
Evidence 1 Activity

Activity

MIC
Antibacterial
5 µg/mL

Target

Escherichia coli D31

Source & Reference

dbAMP DRAMP

Other

Source Activity Label Source Definition
Antibacterial
dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.
Active against E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E.
Evidence 2 Activity

Activity

MIC
Antibacterial
10 µg/mL

Target

Staphylococcus epidermidis

Source & Reference

dbAMP DRAMP

Other

Active against E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (
Evidence 3 Activity

Activity

MIC
Antibacterial
30 µg/mL

Target

Citrobacter freundii

Source & Reference

dbAMP DRAMP

Other

ainst E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa
Evidence 4 Activity

Activity

MIC
Antibacterial
50 µg/mL

Target

Staphylococcus aureus

Source & Reference

dbAMP DRAMP

Other

. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. py
Evidence 5 Activity

Activity

MIC
Antibacterial
80 µg/mL

Target

Candida albicans

Source & Reference

dbAMP DRAMP

Other

MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. A
Evidence 6 Activity

Activity

MIC
Antibacterial
100 µg/mL

Target

S. marcescens

Source & Reference

dbAMP DRAMP

Other

loacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >5
Evidence 7 Activity

Activity

MIC
Antibacterial
>500 µg/mL

Target

C.albicans 315 or B311

Source & Reference

dbAMP DRAMP

Other

S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >5
Evidence 8 Activity

Activity

MIC
Antibacterial
>500 µg/mL

Target

C.glabrata 359

Source & Reference

dbAMP DRAMP

Other

yogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 8072
Evidence 9 Activity

Activity

MIC
Antibacterial
>500 µg/mL

Target

C.krusei 355

Source & Reference

dbAMP DRAMP

Other

e against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof
Evidence 10 Activity

Activity

MIC
Antibacterial
>500 µg/mL

Target

C.neoformans 316

Source & Reference

dbAMP DRAMP

Other

or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof et al., 2000 ). Also active against
Evidence 11 Activity

Activity

MIC
Antibacterial
>500 µg/mL

Target

A.fumigatus X 807219

Source & Reference

dbAMP DRAMP

Other

9 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof et al., 2000 ). Also active against P. falciparum (IC50 ~100 uM) (see ref AP4
Evidence 12 Activity

Activity

IC50
IC50 = >60 µM
Anticancer
>60 µM

Target

Pancreatic Cancer
HeLa
Pancreatic

Assay & Model

WST-1 assay
Not Available

Source & Reference

dbAMP 3.0 CancerPPD 2.0 dbACP SATPdb
CancerPPD2
2011

Other

Source Activity Label Source Definition
Anticancer
CancerPPD 2.0 manually curated experimentally verified anticancer peptide/protein annotation. dbAMP 3.0 classified as Anticancer under the disease-associated peptide function classes.
Evidence 13 Activity

Activity

MIC
Antifungal
5 µg/mL

Target

Escherichia coli D31

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

Source Activity Label Source Definition
Antifungal
dbAMP classified under the Anti-fungal function class. DRAMP antifungal activity supported by activity records against fungal target organisms.
Active against E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E.
Evidence 14 Activity

Activity

MIC
Antifungal
10 µg/mL

Target

Staphylococcus epidermidis

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

Active against E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (
Evidence 15 Activity

Activity

MIC
Antifungal
30 µg/mL

Target

Citrobacter freundii

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

ainst E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa
Evidence 16 Activity

Activity

MIC
Antifungal
50 µg/mL

Target

Staphylococcus aureus

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. py
Evidence 17 Activity

Activity

MIC
Antifungal
80 µg/mL

Target

Candida albicans

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. A
Evidence 18 Activity

Activity

MIC
Antifungal
100 µg/mL

Target

S. marcescens

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

loacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >5
Evidence 19 Activity

Activity

MIC
Antifungal
>500 µg/mL

Target

C.albicans 315 or B311

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >5
Evidence 20 Activity

Activity

MIC
Antifungal
>500 µg/mL

Target

C.glabrata 359

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

yogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 8072
Evidence 21 Activity

Activity

MIC
Antifungal
>500 µg/mL

Target

C.krusei 355

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

e against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof
Evidence 22 Activity

Activity

MIC
Antifungal
>500 µg/mL

Target

C.neoformans 316

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof et al., 2000 ). Also active against
Evidence 23 Activity

Activity

MIC
Antifungal
>500 µg/mL

Target

A.fumigatus X 807219

Source & Reference

dbAMP DRAMP CAFPdb Antifungipept

Other

9 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof et al., 2000 ). Also active against P. falciparum (IC50 ~100 uM) (see ref AP4
Evidence 24 Activity

Activity

MIC
Antiparasitic
5 µg/mL

Target

Escherichia coli D31

Source & Reference

dbAMP 3.0 DRAMP

Other

Source Activity Label Source Definition
Antiparasitic
DRAMP antiparasitic activity supported by activity records against parasitic targets. dbAMP 3.0 classified under the Anti-parasitic function class.
Active against E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E.
Evidence 25 Activity

Activity

MIC
Antiparasitic
10 µg/mL

Target

Staphylococcus epidermidis

Source & Reference

dbAMP 3.0 DRAMP

Other

Active against E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (
Evidence 26 Activity

Activity

MIC
Antiparasitic
30 µg/mL

Target

Citrobacter freundii

Source & Reference

dbAMP 3.0 DRAMP

Other

ainst E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa
Evidence 27 Activity

Activity

MIC
Antiparasitic
50 µg/mL

Target

Staphylococcus aureus

Source & Reference

dbAMP 3.0 DRAMP

Other

. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. py
Evidence 28 Activity

Activity

MIC
Antiparasitic
80 µg/mL

Target

Candida albicans

Source & Reference

dbAMP 3.0 DRAMP

Other

MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. A
Evidence 29 Activity

Activity

MIC
Antiparasitic
100 µg/mL

Target

S. marcescens

Source & Reference

dbAMP 3.0 DRAMP

Other

loacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >5
Evidence 30 Activity

Activity

MIC
Antiparasitic
>500 µg/mL

Target

C.albicans 315 or B311

Source & Reference

dbAMP 3.0 DRAMP

Other

S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >5
Evidence 31 Activity

Activity

MIC
Antiparasitic
>500 µg/mL

Target

C.glabrata 359

Source & Reference

dbAMP 3.0 DRAMP

Other

yogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 8072
Evidence 32 Activity

Activity

MIC
Antiparasitic
>500 µg/mL

Target

C.krusei 355

Source & Reference

dbAMP 3.0 DRAMP

Other

e against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof
Evidence 33 Activity

Activity

MIC
Antiparasitic
>500 µg/mL

Target

C.neoformans 316

Source & Reference

dbAMP 3.0 DRAMP

Other

or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof et al., 2000 ). Also active against
Evidence 34 Activity

Activity

MIC
Antiparasitic
>500 µg/mL

Target

A.fumigatus X 807219

Source & Reference

dbAMP 3.0 DRAMP

Other

9 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof et al., 2000 ). Also active against P. falciparum (IC50 ~100 uM) (see ref AP4
Evidence 35 Activity

Activity

MIC
Antiviral
5 µg/mL

Target

Escherichia coli D31

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

Source Activity Label Source Definition
Antiviral
DRAMP antiviral activity supported by activity records against viral targets. dbAMP 3.0 classified under the Anti-viral function class.
Active against E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E.
Evidence 36 Activity

Activity

MIC
Antiviral
10 µg/mL

Target

Staphylococcus epidermidis

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

Active against E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (
Evidence 37 Activity

Activity

MIC
Antiviral
30 µg/mL

Target

Citrobacter freundii

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

ainst E. coli D31 (MIC 5 ug/ml), K. pneumoniae, P. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa
Evidence 38 Activity

Activity

MIC
Antiviral
50 µg/mL

Target

Staphylococcus aureus

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

. putida, S. epidermidis (MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. py
Evidence 39 Activity

Activity

MIC
Antiviral
80 µg/mL

Target

Candida albicans

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

MIC 10 ug/ml), C. freundii (MIC 30 ug/ml), E. cloacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. A
Evidence 40 Activity

Activity

MIC
Antiviral
100 µg/mL

Target

S. marcescens

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

loacae, E. coli, S. aureus (MIC 50 ug/ml), C. albicans (MIC 80 ug/ml), P. aeruginosa, and S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >5
Evidence 41 Activity

Activity

MIC
Antiviral
>500 µg/mL

Target

C.albicans 315 or B311

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

S. marcescens (MIC 100 ug/ml). Also S. pyogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >5
Evidence 42 Activity

Activity

MIC
Antiviral
>500 µg/mL

Target

C.glabrata 359

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

yogenes, and S. cerevisiae. Active against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 8072
Evidence 43 Activity

Activity

MIC
Antiviral
>500 µg/mL

Target

C.krusei 355

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

e against fungi C.albicans 315 or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof
Evidence 44 Activity

Activity

MIC
Antiviral
>500 µg/mL

Target

C.neoformans 316

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

or B311 (MIC >500 ug/ml), C.glabrata 359 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof et al., 2000 ). Also active against
Evidence 45 Activity

Activity

MIC
Antiviral
>500 µg/mL

Target

A.fumigatus X 807219

Source & Reference

dbAMP 3.0 DRAMP DRAVP 2.0

Other

9 (MIC >500 ug/ml), C.krusei 355 (MIC >500 ug/ml), C.neoformans 316 (MIC >500 ug/ml), and A.fumigatus X 807219 (MIC>500 ug/ml) ( van't Hof et al., 2000 ). Also active against P. falciparum (IC50 ~100 uM) (see ref AP4
Evidence 46 Activity

Activity

Cell-penetrating Peptides

Target

Monomac6 Human Monocytes

Source & Reference

CPPsite3.0
CPPsite3

Other

Source Activity Label Source Definition
Cell-penetrating Peptides
CPPsite3.0 experimentally validated cell-penetrating peptide annotation.
Natural residues Linear L Cationic
Protein derived
No difference in cellular uptake slightly between Cf-labelled and non-labelled
In vitro
CCSCSSCCSCCCC
Evidence 47 Activity

Activity

50% Hemolysis
Toxicity
19±1.0 µM
Hemolytic Cytotoxic
50% Hemolysis | 19±1.0 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
A critical comparison of the hemolytic and fungicidal activities of cationic antimicrobial peptides | FEBS Lett | 1999
Evidence 48 Activity

Activity

50% Hemolysis
Toxicity
17±1.0 µM
Hemolytic Cytotoxic
50% Hemolysis | 17±1.0 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
A critical comparison of the hemolytic and fungicidal activities of cationic antimicrobial peptides | FEBS Lett | 1999
Evidence 49 Activity

Activity

50% Hemolysis
Toxicity
8.0±0.1 µM
Hemolytic Cytotoxic
50% Hemolysis | 8.0±0.1 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
A critical comparison of the hemolytic and fungicidal activities of cationic antimicrobial peptides | FEBS Lett | 1999
Evidence 50 Activity

Activity

50% Hemolysis
Toxicity
7.2±3.4 µM
Hemolytic Cytotoxic
50% Hemolysis | 7.2±3.4 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
A critical comparison of the hemolytic and fungicidal activities of cationic antimicrobial peptides | FEBS Lett | 1999
Evidence 51 Activity

Activity

0% Hemolysis
Toxicity
25 µg/mL
Hemolytic Cytotoxic
0% Hemolysis | 25 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
All-D-magainin: chirality, antimicrobial activity and proteolytic resistance | FEBS Lett | 1990
Evidence 52 Activity

Activity

1% Hemolysis
Toxicity
80 µg/mL
Hemolytic Cytotoxic
1% Hemolysis | 80 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
All-D-magainin: chirality, antimicrobial activity and proteolytic resistance | FEBS Lett | 1990
Evidence 53 Activity

Activity

2% Hemolysis
Toxicity
200 µg/mL
Hemolytic Cytotoxic
2% Hemolysis | 200 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
All-D-magainin: chirality, antimicrobial activity and proteolytic resistance | FEBS Lett | 1990
Evidence 54 Activity

Activity

50% Hemolysis
Toxicity
>81 µM
Hemolytic Cytotoxic
50% Hemolysis | >81 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
A critical comparison of the hemolytic and fungicidal activities of cationic antimicrobial peptides | FEBS Lett | 1999
Evidence 55 Activity

Activity

50% Cell death
Toxicity
>500 µM
Cytotoxic
50% Cell death | >500 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Mouse fibroblasts NIH 3T3

Source & Reference

NA
DBAASP
Antimicrobial peptide GW-H1-induced apoptosis of human gastric cancer AGS cell line is enhanced by suppression of autophagy. | Mol Cell Biochem | 2015
Evidence 56 Activity

Activity

Toxicity
Hemolytic Cytotoxic
- | NA | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Peptoid-Substituted Hybrid Antimicrobial Peptide Derived from Papiliocin and Magainin 2 with Enhanced Bacterial Selectivity and Anti-inflammatory Activity. | Biochemistry | 2015
Evidence 57 Activity

Activity

50% Hemolysis
Toxicity
>1000 µM
Hemolytic Cytotoxic
50% Hemolysis | >1000 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Position-dependent hydrophobicity of the antimicrobial magainin peptide affects the mode of peptide-lipid interactions and selective toxicity. | Biochemistry | 2002
Evidence 58 Activity

Activity

50% Hemolysis
Toxicity
>256 µg/mL
Hemolytic Cytotoxic
50% Hemolysis | >256 | µg/mL | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
The zebrafish embryo as a tool for screening and characterizing pleurocidin host-defense peptides as anti-cancer agents. | Dis Model Mech | 2011
Evidence 59 Activity

Activity

30% Hemolysis
Toxicity
150 µM
Hemolytic Cytotoxic
30% Hemolysis | 150 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Peptide hydrophobicity controls the activity and selectivity of magainin 2 amide in interaction with membranes. | Biochemistry | 1997
Evidence 60 Activity

Activity

50% Cell death
Toxicity
236 µM
Cytotoxic
50% Cell death | 236 | µM | target cell: Mouse fibroblasts NIH 3T3

Target

Mouse fibroblasts NIH 3T3

Source & Reference

NA
DBAASP
Antimicrobial peptide GW-H1-induced apoptosis of human gastric cancer AGS cell line is enhanced by suppression of autophagy. | Mol Cell Biochem | 2015
Evidence 61 Activity

Activity

50% Hemolysis
Toxicity
479.5±42.9 µM
Hemolytic Cytotoxic
50% Hemolysis | 479.5±42.9 | µM | target cell: Sheep erythrocytes

Target

Sheep erythrocytes

Source & Reference

NA
DBAASP
A Rapid and Quantitative Flow Cytometry Method for the Analysis of Membrane Disruptive Antimicrobial Activity. | PLoS One | 2016
Evidence 62 Activity

Activity

IC50
Toxicity
132±23.8 µM
Cytotoxic
IC50 | 132±23.8 | µM | target cell: Human PBMC

Target

Human PBMC

Source & Reference

NA
DBAASP
Comparative analysis of internalisation, haemolytic, cytotoxic and antibacterial effect of membrane-active cationic peptides: aspects of experimental setup. | Amino Acids | 2017
Evidence 63 Activity

Activity

50% Hemolysis
Toxicity
>300 µM
Hemolytic Cytotoxic
50% Hemolysis | >300 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Comparative analysis of internalisation, haemolytic, cytotoxic and antibacterial effect of membrane-active cationic peptides: aspects of experimental setup. | Amino Acids | 2017
Evidence 64 Activity

Activity

0% Hemolysis
Toxicity
50 µM
Hemolytic Cytotoxic
0% Hemolysis | 50 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Inducing toxicity by introducing a leucine-zipper-like motif in frog antimicrobial peptide, magainin 2. | Biochem J | 2011
Evidence 65 Activity

Activity

25% Cell death
Toxicity
100 µM
Cytotoxic
25% Cell death | 100 | µM | target cell: Chinese hamster ovary cells CHO-K1

Target

Chinese hamster ovary cells CHO-K1

Source & Reference

NA
DBAASP
Inducing toxicity by introducing a leucine-zipper-like motif in frog antimicrobial peptide, magainin 2. | Biochem J | 2011
Evidence 66 Activity

Activity

50% Hemolysis
Toxicity
>100 µM
Hemolytic Cytotoxic
50% Hemolysis | >100 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence. | Molecules | 2021
Evidence 67 Activity

Activity

0% Hemolysis
Toxicity
64 µM
Hemolytic Cytotoxic
0% Hemolysis | 64 | µM | target cell: Mouse erythrocytes

Target

Mouse erythrocytes

Source & Reference

NA
DBAASP
Antibacterial and Antibiofilm Activity and Mode of Action of Magainin 2 against Drug-Resistant Acinetobacter baumannii. | Int J Mol Sci | 2018
Evidence 68 Activity

Activity

Toxicity
Cytotoxic
NA | target cell: Human keratinocytes HaCat

Target

Human keratinocytes HaCat

Source & Reference

NA
DBAASP
Antibacterial and Antibiofilm Activity and Mode of Action of Magainin 2 against Drug-Resistant Acinetobacter baumannii. | Int J Mol Sci | 2018
Evidence 69 Hemolysis

Source & Reference

APD_complete HemoPI PD_Hemolysis
APD_complete PD_Hemolysis

Other

Source Activity Label Source Definition
Hemolysis
APD_complete APD-derived hemolytic peptide annotation.
Additional Detail Fields 1 fields
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