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Peptide record

TPDB00584

Antibacterial Anticancer Antifungal Antioxidant Antiparasitic Antiviral Cell-penetrating Peptides Hemolysis Neurotoxin Toxicity standard
35 amino acids
Basic Information
3D PDB MODEL
Drag to rotate. Click a residue or atom to inspect it; the selected residue is highlighted in amber.
TPDB00584
Antibacterial Anticancer Antifungal Antioxidant Antiparasitic Antiviral Cell-penetrating Peptides Hemolysis Neurotoxin Toxicity
Anti-infective peptides Cancer-related peptides Toxicity and safety peptides Delivery and barrier-penetrating peptides Functional / health-promoting peptides
AntiBP3 dbAMP DRAMP Peptipedia ACP740 _ACP240 AntiCP 2.0 SATPdb dbACP iACP-DRLF Antifungipept dbAMP 3.0 i2APP APD_complete APD_hemolytic HemoPI Hemolytic2 PD_Hemolysis NTxPred2 ToxinPred 3.0
standard
No
A 35-aa standard natural multi-activity (Antibacterial, Anticancer, Antifungal, and other sources) peptide sequence curated from AntiBP3, dbAMP, DRAMP, and other sources, with an available 3D structural model.
Sequence
GFGALFKFLAKKVAKTVAKQAAKQGAKYVVNKQME
Physicochemical Analysis
C176H289N47O44S1
RDCHIPSW
K
3799.58
10.78
8
1
18
+7
5.68
-0.131
75.43
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
1490
39.21
6
Residue Composition
number
7
A
0
R
1
N
0
D
0
C
1
E
3
Q
3
G
0
H
0
I
2
L
8
K
1
M
3
F
0
P
0
S
1
T
0
W
1
Y
4
V
Amino Acid Distribution
A: 7 R: 0 N: 1 D: 0 C: 0 E: 1 Q: 3 G: 3 H: 0 I: 0 L: 2 K: 8 M: 1 F: 3 P: 0 S: 0 T: 1 W: 0 Y: 1 V: 4
Chemical Descriptors
35
C176H289N47O44S1
3799.58
10.78
+7
5.68
-0.131
Evidence Records 11 records
Evidence 1 Activity

Activity

MIC
Antibacterial
2.5-5 µM

Target

Enterococcus faecalis ATCC 29212

Source & Reference

dbAMP DRAMP

Other

Source Activity Label Source Definition
Antibacterial
dbAMP classified as Antibacterial under the Anti-bacterial function class. DRAMP antibacterial activity supported by MIC records against bacterial target organisms.
63 uM), P. aeruginosa ATCC 27853 (0.63-1.25 uM), S. aureus ATCC 29213 ().63-1.25 uM), and E. faecalis ATCC 29212 (MIC 2.5-5 uM). It is also insecticidal (EC50 5.9 pmol/mg fly). Also: antiparasitic activity against T.
Evidence 2 Activity

Activity

MIC
Antifungal
2.5-5 µM

Target

Enterococcus faecalis ATCC 29212

Source & Reference

dbAMP DRAMP Antifungipept

Other

Source Activity Label Source Definition
Antifungal
dbAMP classified under the Anti-fungal function class. DRAMP antifungal activity supported by activity records against fungal target organisms.
63 uM), P. aeruginosa ATCC 27853 (0.63-1.25 uM), S. aureus ATCC 29213 ().63-1.25 uM), and E. faecalis ATCC 29212 (MIC 2.5-5 uM). It is also insecticidal (EC50 5.9 pmol/mg fly). Also: antiparasitic activity against T.
Evidence 3 Activity

Activity

MIC
Antiparasitic
2.5-5 µM

Target

Enterococcus faecalis ATCC 29212

Source & Reference

dbAMP 3.0 DRAMP

Other

Source Activity Label Source Definition
Antiparasitic
DRAMP antiparasitic activity supported by activity records against parasitic targets. dbAMP 3.0 classified under the Anti-parasitic function class.
63 uM), P. aeruginosa ATCC 27853 (0.63-1.25 uM), S. aureus ATCC 29213 ().63-1.25 uM), and E. faecalis ATCC 29212 (MIC 2.5-5 uM). It is also insecticidal (EC50 5.9 pmol/mg fly). Also: antiparasitic activity against T.
Evidence 4 Activity

Activity

MIC
Antiviral
2.5-5 µM

Target

Enterococcus faecalis ATCC 29212

Source & Reference

dbAMP 3.0 DRAMP

Other

Source Activity Label Source Definition
Antiviral
DRAMP antiviral activity supported by activity records against viral targets. dbAMP 3.0 classified under the Anti-viral function class.
63 uM), P. aeruginosa ATCC 27853 (0.63-1.25 uM), S. aureus ATCC 29213 ().63-1.25 uM), and E. faecalis ATCC 29212 (MIC 2.5-5 uM). It is also insecticidal (EC50 5.9 pmol/mg fly). Also: antiparasitic activity against T.
Evidence 5 Activity

Activity

EC50
Hemolysis
24.4 µM
[Ref.11792701]EC50=24.4 µM against human red blood cells.

Target

human red blood cells

Source & Reference

Hemolytik APD_complete APD_hemolytic PD_Hemolysis
DRAMP4
J Biol Chem. 2002 Mar 29 277(13):11208-11216.##Biochemistry. 2007 Mar 20 46(11):3576-3585.

Other

Source Activity Label Source Definition
Hemolysis
APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation. Hemolytik experimentally validated hemolytic peptide annotation.
Evidence 6 Activity

Activity

Neurotoxin
FUNCTION: Has antimicrobial activity against B.subtilis, E.coli, E.faecalis, P.denitrificans, P.aeruginosa, P.putida, S.aureus, and S.epidermidis. Shows insecticidal and hemolytic activities. Probably acts by disturbing membrane function with its amphipathic structure. Synergistically increases the insecticidal activity of CSTX-1 (AC P81694), CSTX-9 (AC P58604), and CSTX-13 (AC P83919) by up to 65% (PubMed:15914655). Also inhibits the formation of nitric oxide by neuronal nitric oxide synthase. {ECO:0000269|PubMed:10669026, ECO:0000269|PubMed:11792701, ECO:0000269|PubMed:15914655, ECO:0000269|PubMed:17313650}.

Source & Reference

NA
UniProtKB/Swiss-Prot Tox-Prot

Other

Cupiennius salei (American wandering spider)
Neurotoxin
Has antimicrobial activity against B.subtilis, E.coli, E.faecalis, P.denitrificans, P.aeruginosa, P.putida, S.aureus, and S.epidermidis. Shows insecticidal and hemolytic activities.
Cupiennin-1a (Cu-1a) (M-ctenitoxin-Cs1a) (M-CNTX-Cs1a)
spider
Cationic peptide 04 (cupiennin) family, 01 subfamily
Evidence at protein level
3D-structure Amidation Antibiotic Antimicrobial Cytolysis Direct protein sequencing Hemolysis Neurotoxin Secreted Toxin
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:10669026}.
Evidence 7 Activity

Activity

50% Hemolysis
Toxicity
7.8 µM
Hemolytic Cytotoxic
50% Hemolysis | 7.8 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
N-terminal aromatic residues closely impact the cytolytic activity of cupiennin 1a, a major spider venom peptide. | Toxicon | 2013
Evidence 8 Activity

Activity

EC50
Toxicity
0.56 µM
Cytotoxic
EC50 | 0.56 | µM | target cell: Human lymphocytes

Target

Human lymphocytes

Source & Reference

NA
DBAASP
Cupiennin 1a exhibits a remarkably broad, non-stereospecific cytolytic activity on bacteria, protozoan parasites, insects, and human cancer cells. | Amino Acids | 2011
Evidence 9 Activity

Activity

50% Hemolysis
Toxicity
20.5 µM
Hemolytic Cytotoxic
50% Hemolysis | 20.5 | µM | target cell: Human erythrocytes

Target

Human erythrocytes

Source & Reference

NA
DBAASP
Evidence 10 Activity

Activity

EC50
Toxicity
24.4 µM
Hemolytic
Hemolytic_activity: [Ref.11792701]EC50=24.4 µM against human red blood cells.

Target

erythrocytes

Source & Reference

NA
DRAMP
J Biol Chem. 2002 Mar 29 277(13):11208-11216.##Biochemistry. 2007 Mar 20 46(11):3576-3585.
Evidence 11 Hemolysis

Activity

24.4 µM
EC50
[Ref.11792701]EC50=24.4 µM against human red blood cells.
Hemolysis

Target

human red blood cells

Source & Reference

APD_complete APD_hemolytic HemoPI PD_Hemolysis
J Biol Chem. 2002 Mar 29 277(13):11208-11216.##Biochemistry. 2007 Mar 20 46(11):3576-3585.
DRAMP4
APD_complete APD_hemolytic PD_Hemolysis

Other

Source Activity Label Source Definition
Hemolysis
APD_complete APD-derived hemolytic peptide annotation. APD_hemolytic APD hemolytic peptide annotation.
Additional Detail Fields 7 fields
3D-structure Amidation Antibiotic Antimicrobial Cytolysis Direct protein sequencing Hemolysis Neurotoxin Secreted Toxin
Neurotoxin
Evidence at protein level
Cationic peptide 04 (cupiennin) family, 01 subfamily
Cupiennin-1a (Cu-1a) (M-ctenitoxin-Cs1a) (M-CNTX-Cs1a)
TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|PubMed:10669026}.
spider