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Peptide record

TPDB00574

Antioxidative peptide PI Antioxidant standard
9 amino acids
Basic Information
3D PDB MODEL
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TPDB00574
Antioxidative peptide PI
Antioxidant
Functional / health-promoting peptides
AODB Multi-AOP
standard
No
A 9-aa standard natural antioxidant peptide sequence curated from AODB and Multi-AOP, with an available 3D structural model.
Sequence
GEPGPPGAP
Physicochemical Analysis
C34H51N9O12
RNDCQHILKMFSTWYV
P
777.83
3.29
0
1
1
-1
3.68
-1.033
11.11
Mammalian: 30 hour Yeast: >20 hour E.coli: >10 hour
0
0.00
0
Residue Composition
number
1
A
0
R
0
N
0
D
0
C
1
E
0
Q
3
G
0
H
0
I
0
L
0
K
0
M
0
F
4
P
0
S
0
T
0
W
0
Y
0
V
Amino Acid Distribution
A: 1 R: 0 N: 0 D: 0 C: 0 E: 1 Q: 0 G: 3 H: 0 I: 0 L: 0 K: 0 M: 0 F: 0 P: 4 S: 0 T: 0 W: 0 Y: 0 V: 0
Chemical Descriptors
9
C34H51N9O12
777.83
3.29
-1
3.68
-1.033
826 Da
3.34
0.00
NCC(=O)N[C@@]([H])(CCC(=O)O)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N1[C@@]([H])(CCC1)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N[C@@]([H])(C)C(=O)N1[C@@]([H])(CCC1)C(=O)O
777.83 Da
Evidence Records 1 records
Evidence 1 Activity

Activity

Antioxidative activity
(1) The bioactive sequence of the peptide is Gly-Glu-Hyp-Gly-Pro-Hyp-Gly-Ala-Hyp. Hydroxyproline (Hyp) is a product of hydroxylation of proline. (2) The antioxidative activity of the purified peptides in a water/alcohol system was examined by the TBA test and the peptide showed potent antioxidative activity (MDA concentartion, 2.26 × 10^-7 M).
Literature report: N.D Prediction: ToxinPred
Antioxidant

Assay & Model

Enzymatic hydrolysis
The bovine gelatin was hydrolyzed with serial digestions in the order of Alcalase, Pronase E, and collagenase using a three-step recycling membrane reactor. The second enzymatic hydrolysate (hydrolyzed with Pronase E) showed the highest antioxidative activity, as determined by the thiobarbituric acid method.

Source & Reference

AODB
Ertani A, Francioso O, Ferrari E, Schiavon M, Nardi S. Spectroscopic-Chemical Fingerprint and Biostimulant Activity of a Protein-Based Product in Solid Form. Molecules. 2018 Apr 27 23(5):1031.
10.3390/molecules23051031
[1] Kim S K, Kim Y T, Byun H G, et al. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska pollack skin[J]. Journal of Agricultural & Food Chemistry, 2001, 49(4):1984.
2018
DFBP

Other

Native peptide
Animal
Bovine
The gelatin hydrolysate of bovine skin