TPDB

3D PDB MODEL

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TPDB ID TPDB00546

Peptide_Name

ACE inhibitor FY Hypotensive peptide

Activity

ACE inhibitors Antihypertensive Antioxidant Antiparasitic Bitter Neuropeptide iDPPIV Selfassembly

Activity_category_statistics

Anti-infective peptides Cardiometabolic peptides Neuroactive peptides Food sensory peptides Functional / health-promoting peptides Self-assembling and biomaterial peptides

Database_Source

FermFooDB Peptipedia AHTPDB AHTpin Tastepeptides-Meta iDPPIV SAPdb

Sequence_Type

standard

Has_Modification No

Description A 2-aa standard natural multi-activity (ACE inhibitors, Antihypertensive, Antioxidant, and other sources) peptide sequence curated from FermFooDB, Peptipedia, AHTPDB, and other sources, with an available 3D structural model.

Sequence

FY

FASTA

>TPDB00546|ACE inhibitors;Antihypertensive;Antioxidant;Antiparasitic;Bitter;Neuropeptide;iDPPIV;Selfassembly|length=2
FY

Formula C18H20N2O4

Absent Amino Acids ARNDCEQGHILKMPSTWV

Common Amino Acids FY

Mass 328.37

PI 5.94

Basic Residues 0

Acidic Residues 0

Hydrophobic Residues 2

Net Charge -0

Boman Index -2.07

Hydrophobicity 0.750

Aliphatic Index 0.00

Half Life

Mammalian: 1.1 hour Yeast: 3 min E.coli: 2 min

Extinction Coefficient Cystines 1490

Absorbance 280nm 453.76

Polar Residues 1

number

0

A

0

R

0

N

0

D

0

C

0

E

0

Q

0

G

0

H

0

I

0

L

0

K

0

M

1

F

0

P

0

S

0

T

0

W

1

Y

0

V

Amino Acid Distribution

A: 0 R: 0 N: 0 D: 0 C: 0 E: 0 Q: 0 G: 0 H: 0 I: 0 L: 0 K: 0 M: 0 F: 1 P: 0 S: 0 T: 0 W: 0 Y: 1 V: 0

Length

2

Formula

C18H20N2O4

Mass

328.37

PI

5.94

Net Charge

-0

Boman Index

-2.07

Hydrophobicity

0.750

Chemical_Mass

328.3616

Monoisotopic_Mass

328.1418

Record 1 Selfassembly

Source

PMID 25198430

DOI 10.1016/j.compbiomed.2021.104391

Title

SAPdb: A database of short peptides and the corresponding nanostructures formed by self-assembly

Self-Assembly

Activity Endpoint Nanostructure formation

Nanostructure Flower like microstructure

Assembly Trigger temperature: ~64.7 Â°C solvent: Methanol + HFIP (1,1,1,3,3,3-hexafluoro-2-propanol) concentration: 1mg/ml

Source Annotation

Selfassembly; Nanostructure=Flower like microstructure; Trigger=temperature: ~64.7 Â°C; solvent: Methanol + HFIP (1,1,1,3,3,3-hexafluoro-2-propanol); concentration: 1mg/ml

Assembly Evidence Scanning Electron Microscopy (SEM)

Source Activity Label Selfassembly

Evidence 1 Activity

Activity

Activity Endpoint IC50

Detail Source Activity ACE inhibitors

Activity Measurement 25.00 µM

Function Inhibitor of Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) (MEROPS ID: M02-001)

Detail Activity ACE inhibitor

Activity Code ah

Source & Reference

Database Source FermFooDB

Authors Yano S., Suzuki K., Funatsu G.

Title

Isolation from alpha-zein of thermolysin peptides with angiotensin I-converting enzyme inhibitory activity. Biosci. Biotech. Biochem. 60, 661-663 (1996)

Year 1996

Literature Source Journal

Other

Number of Residues 2

Additional Info

BIOPEP-UWM database of bioactive peptides SMILES: N[C@@H](CC1=CC=C(C=C1))C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O InChI: InChI=1S/C18H20N2O4/c19-15(10-12-4-2-1-3-5-12)17(22)20-16(18(23)24)11-13-6-8-14(21)9-7-13/h1-9,15-16,21H,10-11,19H2,(H,20,22)(H,23,24)/t15-,16-/m0/s1 InChIKey=FSXRLASFHBWESK-HOTGVXAUSA-N Information concerning Angiotensin-Converting Enzyme (ACE) is available in MEROPS database of proteolytic enzymes (http://merops.sanger.ac.uk/) ID: M02-001 Another value of peptide IC50 = 3.7 Loponen J., 2004, Angiotensin converting enzyme inhibitory peptides in Finnish cereals: a database survey. Agric. Food Sci., 13, 39-45 Found in amaranth (Amarantus hypochondriacus) protein hydrolysate: Barba de la Rosa A. P., Barba Montoya A., Martínez-Cuevas P., Hernández-Ledesma B., León-Galván M. F., De León-Rodríguez A., González C., 2010, Tryptic amaranth glutelin digests induce endothelial nitric oxide production through inhibition of ACE: antihypertensive role of amaranth peptides. Nitric Oxide, 23, 106-111 Inhibitor of alpha-amylase (EC 3.2.1.1) according to the BIOPEP-UWM database of bioactive peptides Inhibitor of alpha-glucosidase (EC 3.2.1.20) according to the BIOPEP-UWM database of bioactive peptides Hypotensive peptide according to the BIOPEP-UWM database of bioactive peptides the DFBP database Antioxidative peptide according to the BIOPEP-UWM database of bioactive peptides the PlantpepDB database Bitter peptide according to the BIOPEP-UWM database of sensory peptides and amino acids (ID 478) the ChEMBL database (ID CHEMBL54572) the PubChem database (ID 515709)

Evidence 2 Activity

Activity

Activity Endpoint IC50

Detail Source Activity Antihypertensive

Activity Measurement 25.00 µM

Function Inhibitor of Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) (MEROPS ID: M02-001)

Detail Activity ACE inhibitor

Activity Code ah

Source & Reference

Database Source AHTPDB FermFooDB

Authors Yano S., Suzuki K., Funatsu G.

Title

Isolation from alpha-zein of thermolysin peptides with angiotensin I-converting enzyme inhibitory activity. Biosci. Biotech. Biochem. 60, 661-663 (1996)

Year 1996

Literature Source Journal

Other

Source Activity Label	Source Definition
Antihypertensive	AHTPDB manually curated experimentally validated antihypertensive peptide annotation.

Number of Residues 2

Additional Info

BIOPEP-UWM database of bioactive peptides SMILES: N[C@@H](CC1=CC=C(C=C1))C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O InChI: InChI=1S/C18H20N2O4/c19-15(10-12-4-2-1-3-5-12)17(22)20-16(18(23)24)11-13-6-8-14(21)9-7-13/h1-9,15-16,21H,10-11,19H2,(H,20,22)(H,23,24)/t15-,16-/m0/s1 InChIKey=FSXRLASFHBWESK-HOTGVXAUSA-N Information concerning Angiotensin-Converting Enzyme (ACE) is available in MEROPS database of proteolytic enzymes (http://merops.sanger.ac.uk/) ID: M02-001 Another value of peptide IC50 = 3.7 Loponen J., 2004, Angiotensin converting enzyme inhibitory peptides in Finnish cereals: a database survey. Agric. Food Sci., 13, 39-45 Found in amaranth (Amarantus hypochondriacus) protein hydrolysate: Barba de la Rosa A. P., Barba Montoya A., Martínez-Cuevas P., Hernández-Ledesma B., León-Galván M. F., De León-Rodríguez A., González C., 2010, Tryptic amaranth glutelin digests induce endothelial nitric oxide production through inhibition of ACE: antihypertensive role of amaranth peptides. Nitric Oxide, 23, 106-111 Inhibitor of alpha-amylase (EC 3.2.1.1) according to the BIOPEP-UWM database of bioactive peptides Inhibitor of alpha-glucosidase (EC 3.2.1.20) according to the BIOPEP-UWM database of bioactive peptides Hypotensive peptide according to the BIOPEP-UWM database of bioactive peptides the DFBP database Antioxidative peptide according to the BIOPEP-UWM database of bioactive peptides the PlantpepDB database Bitter peptide according to the BIOPEP-UWM database of sensory peptides and amino acids (ID 478) the ChEMBL database (ID CHEMBL54572) the PubChem database (ID 515709)

Evidence 3 Activity

Activity

Activity Endpoint EC50

Detail Source Activity Antihypertensive

Activity Measurement 0.00 µM

Function Lowering blood pressure in rats

Detail Activity hypotensive

Activity Code hyp

Source & Reference

Database Source AHTPDB FermFooDB

Authors Sato M., Hosokawa T., Yamaguchi T., Nakano T., Muramoto K., Kahara T., Funayama K., Kobayashi A., Nakano T.

Title

Angiotensin I-converting enzyme inhibitory peptides derived from wakame (Undaria pinnatifida) and their antihypertensive effect in spontaneously hypertensive rats. J. Agric. Food. Chem., 50, 6245-6252, 2002

Year 2002

Literature Source Journal

Other

Number of Residues 2

Additional Info

BIOPEP-UWM database of bioactive peptides SMILES: N[C@@H](CC1=CC=C(C=C1))C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O InChI: InChI=1S/C18H20N2O4/c19-15(10-12-4-2-1-3-5-12)17(22)20-16(18(23)24)11-13-6-8-14(21)9-7-13/h1-9,15-16,21H,10-11,19H2,(H,20,22)(H,23,24)/t15-,16-/m0/s1 InChIKey: FSXRLASFHBWESK-HOTGVXAUSA-N Inhibitor of alpha-glucosidase (EC 3.2.1.20) according to the BIOPEP-UWM database of bioactive peptides Inhibitor of alpha-amylase (EC 3.2.1.1) according to the BIOPEP-UWM database of bioactive peptides Inhibitor of Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) (MEROPS ID: M02-001) according to the AHTPDB database the BIOPEP-UWM database of bioactive peptides (ID 3556) the BRENDA database the ChEMBL database the DFBP database the EROP-Moscow database the PlantPepDB database Bitter peptide according to the BIOPEP-UWM database of sensory peptides and amino acids (ID 478) the ChEMBL database (ID CHEMBL54572) the PubChem database (ID 515709)

Evidence 4 Selfassembly

Activity

Activity Endpoint Nanostructure formation

Activity Raw

Selfassembly Nanostructure=Flower like microstructure Trigger=temperature: ~64.7 Â°C solvent: Methanol + HFIP (1,1,1,3,3,3-hexafluoro-2-propanol) concentration: 1mg/ml

Detail Activity Flower like microstructure

Source & Reference

Database Source SAPdb

DOI 10.1016/j.compbiomed.2021.104391

Other

Modification None specified

Assembly Trigger temperature: ~64.7 Â°C solvent: Methanol + HFIP (1,1,1,3,3,3-hexafluoro-2-propanol) concentration: 1mg/ml

Evidence Text

Scanning Electron Microscopy (SEM)

PMID 25198430

Reference Title SAPdb: A database of short peptides and the corresponding nanostructures formed by self-assembly

Notes SAPdb ID 1234 nanostr_class=Other but nanostr gives explicit morphology low-detail seed row.

Assembly_Trigger

temperature: ~64.7 Â°C solvent: Methanol + HFIP (1,1,1,3,3,3-hexafluoro-2-propanol) concentration: 1mg/ml

Modification

None specified

Notes

SAPdb ID 1234 nanostr_class=Other but nanostr gives explicit morphology low-detail seed row.

Number_of_Residues

2

PMID

25198430

Reference_Title

SAPdb: A database of short peptides and the corresponding nanostructures formed by self-assembly